FastQCFastQC Report
Sun 21 May 2023
EGAF00007833186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184723
Sequences flagged as poor quality0
Sequence length235-238
%GC66

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG12639768.42515550310465No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT86224.667529219425843No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT80944.3816958364686585No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACTTGG51842.80636412357963No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT49072.656409867747925No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG28241.528775517937669No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG25221.3652874845038248No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT16230.8786128419308912No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA11130.602523778847247No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA11040.5976516189104768No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT8440.45690033184822676No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT7970.43145682995620466No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT6310.34159254667799893No Hit
GCTGCGTTCTTCATCGATTCGAAACGCCTAGGCATCCGCCGTATGCCCTT5870.31777309809823356No Hit
GCTGCGTTCTTCATCGATGCCTGCGGATGCAATCGCCTCGACACTCGGGT5170.2798785208122432No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4890.26472068989784703No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4350.23548773027722592No Hit
GCTGCGTTCTTCATCGATGCTGCGGCAGTCCTCCTCCCGCTTTTTGTCCG4010.21708179273831626No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC3730.20192396182392014No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCG3640.19705180188714996No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3380.18297667318092495No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG3110.16836019337061436No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3000.16240533122567302No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGGT2820.15266101135213264No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG2520.13642047822956535No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGCTT2220.12017994510699806No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA2200.1190972428988269No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT2200.1190972428988269No Hit
GCTGCGTTCTTCATCGATGCGCACGGCGACCTTGACGCTGACCTTTTCGA2190.11855589179474131No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2150.11639048737839902No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG2130.11530778517022786No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG2080.11260102964979997No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG2060.11151832744162882No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCAGTTGCCGAGAGTCG1850.10014995425583169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)