FastQCFastQC Report
Sun 21 May 2023
EGAF00007833199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325778
Sequences flagged as poor quality0
Sequence length247-250
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC22456568.9319106876462No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA4277313.129493090386704No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG118663.6423576791557446No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC35721.096452185230433No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC29720.912277686031592No Hit
GGAAGTAAAAGTCGTAACAAGGTAGACGAAGAAGGGCTGATCTCGATGCT25270.775681599125785No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC13740.4217596031653457No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC10800.3315140985579137No Hit
GGAAGTAAAAGTCGTAACAACAGGGGAGCGGTCAGCTAATCGACGCAATC10260.31493839363001797No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT10190.31278969113936483No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC9470.2906887512355039No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG8560.26275561885701304No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG8460.2596860438703657No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC7480.22960420900122167No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA5990.1838675417001762No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC5940.18233275420685252No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG5540.1700544542602631No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC5280.16207355929498002No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA4880.1497952593483906No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC4770.14641872686307855No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA4610.1415074068844428No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGGGCTTCGACCTTCATGCCGCGT4440.13628912940714227No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC3990.12247604196722922No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA3820.11725776448992874No Hit
GGAAGTAAAAGTCGTAACAAGGTCGAGCAGAACAAGCTCACCGTCACGGG3820.11725776448992874No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC3750.11510906199927558No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3700.1135742745059519No Hit
GGAAGTAAAAGTCGTAACAAGGGTGAAATCGTCGAGGTTTCGGAAGGCTA3650.11203948701262822No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACCTGCGGAAGGATC3430.10528642204200407No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC3400.10436554954600986No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC3330.10221684705535672No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC3300.10129597455936251No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC3280.10068205956203305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)