FastQCFastQC Report
Sun 21 May 2023
EGAF00007833202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138293
Sequences flagged as poor quality0
Sequence length235-238
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG10285674.37542030326915No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT83006.001749907804444No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT44093.188158475121662No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT20831.5062222961393563No Hit
GCTGCGTTCTTCATCGATGCTGTGGTGGTGACGGTGGCTGCTGCGCTGGG19821.43318895388776No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT14521.0499446826665124No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG5900.42663041513308697No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT5770.41723008395218847No Hit
GCTGCGTTCTTCATCGATGCCAGCTCGCTTACAGTTACAAATTCGGTAAG5520.39915252398892204No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG5010.3622743016638586No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4510.3261191817373258No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4240.3065954169769981No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG3380.2444086107033617No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT3030.21910002675478873No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG2930.2118690027694822No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT2920.21114590037095154No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG2870.20753038837829826No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA2840.2053610811827063No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG2550.18439111162531727No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2470.17860629243707202No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG2460.17788319003854136No Hit
GCTGCGTTCTTCATCGATGCGAGAGACGAGATATCCGTTGCCGAGAGTCG2420.17499078044441874No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG2150.15546701568409102No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG2050.14823599169878449No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG2020.1460666845031925No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCTGAGAGTCG1960.14172807011200855No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1910.1381125581193553No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG1780.12871222693845674No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG1710.12365051014874218No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG1690.12220430535168085No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAAAACAGGAACAACACACACGTGT1650.11931189575755824No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG1650.11931189575755824No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCGAGATATCCGTTGCCGAGAGTCG1560.11280397417078232No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG1550.11208087177225166No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCAGTTGCCGAGAGTCG1540.11135776937372102No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG1510.10918846217812905No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATACGTTGCCGAGAGTCG1470.10629605258400641No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCTAGAGTCG1440.10412674538841445No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG1440.10412674538841445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)