FastQCFastQC Report
Sun 21 May 2023
EGAF00007833210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76301
Sequences flagged as poor quality0
Sequence length236-238
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4766562.46969240245869No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT55777.309209577856122No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG44955.891141662625653No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG14321.8767774996395852No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG8791.1520163562731813No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT8511.115319589520452No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT5190.6802007837380899No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT5120.6710265920499077No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA4920.6448146157979581No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4480.5871482680436692No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT3660.4796791654106762No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG3610.47312617134768875No Hit
GCTGCGTTCTTCATCGATGCGCAAAGGTCACTGGCTGCGTTCTTCATCGA3410.4469141950957392No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT3170.4154598235933998No Hit
GCTGCGTTCTTCATCGATGCTGTCCGCGTAATCGTAGATTGTCTTATGAC3140.41152802715560743No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG3040.3984220390296327No Hit
GCTGCGTTCTTCATCGATGCGATAGCCGAGATATCCGTTGCCGAGAGTCG2940.3853160509036579No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCG2390.3132331162107967No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGGCG2390.3132331162107967No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT2030.2660515589572876No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT1850.24246078033053303No Hit
GCTGCGTTCTTCATCGATGCGCAAAGGTCACTTGCTGCGTTCTTCATCGA1830.2398395827053381No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1810.2372183850801431No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCATTGCCGAGAGTCG1780.2332865886423507No Hit
GCTGCGTTCTTCATCGATGCGCAAAGGTCACTCGCTGCGTTCTTCATCGA1730.22673359457936332No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT1730.22673359457936332No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1580.20707461239040117No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG1550.20314281595260872No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCTAGAGTCG1510.19790042070221883No Hit
GCTGCGTTCTTCATCGATGCAAAGGTCACTGGCTGCGTTCTTCATCGATG1420.18610503138884157No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT1380.18086263613845166No Hit
GCTGCGTTCTTCATCGATGCGCAAAGGTCACTGGCTGCGTTCTTCATCGG1340.17562024088806177No Hit
GCTGCGTTCTTCATCGATGCGAGAGACGAGATATCCGTTGCCGAGAGTCG1320.1729990432628668No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1320.1729990432628668No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG1310.17168844445026932No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1310.17168844445026932No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1290.16906724682507437No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG1290.16906724682507437No Hit
GCTGCGTTCTTCATCGATGCGCAAAGGTCACTAGCTGCGTTCTTCATCGA1280.16775664801247692No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1200.15727185751169712No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCAGAGAGTCG1200.15727185751169712No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1200.15727185751169712No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTA1090.14285527057312486No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1090.14285527057312486No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT1060.13892347413533243No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGTCGAGAGTCG1010.13237048007234506No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1000.13105988125974757No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG970.12712808482195515No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT810.10615850382039553No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCTGAGAGTCG800.10484790500779806No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG800.10484790500779806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)