FastQCFastQC Report
Sun 21 May 2023
EGAF00007833216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80034
Sequences flagged as poor quality0
Sequence length236-238
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3673345.89674388384936No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1270515.874503336082165No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1048113.095684334157983No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT12091.5106079916035684No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTTCACTTCGCAAATCAAAATAA10611.3256865832021392No Hit
GCTGCGTTCTTCATCGCAGCTTCGACAGTGAAGCAGTTCAGGTCGACAAT10521.314441362420971No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA7680.9595921733263364No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG6950.8683809381013069No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA6940.8671314691256217No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT6730.8408926206362296No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG6110.7634255441437389No Hit
GCTGCGTTCTTCATCGATGCCATGAATCCCTGCAGATATCCAACCAGCTC4890.6109903291101282No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC4660.5822525426693655No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4530.5660094459854561No Hit
GCTGCGTTCTTCATCGATGCCTGCAACAGCGCGGCTAGTTTCATTACCAC4270.5335232526176374No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT3340.41732263787890145No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3070.38358697553539745No Hit
GCTGCGTTCTTCATCGATGCGTCCGTACCTTGGCTGCGTTCTTCATCGAT3070.38358697553539745No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT2970.37109228577854414No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT2940.3673438788514881No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2880.3598470649973761No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG2390.29862308518879477No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2020.2523927330884374No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT1990.2486443261613814No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG1970.24614538821001075No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG1940.24239698128295473No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1900.2373991053802134No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1510.1886698153284854No Hit
GCTGCGTTCTTCATCGATGCACCCCAAATCTTGCTTAGGCGCACCGCATG1490.18617087737711474No Hit
GCTGCGTTCTTCATCGATGCTTCGACAGTGAAGCAGTTCAGGTCGACAAT1410.17617512557163206No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1380.17242671864457607No Hit
GCTGCGTTCTTCATCGATGCTGGCTCCAGGGAGCTTCTTGATGAGGTCAG1320.16492990479046404No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG1300.1624309668390934No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG1280.15993202888772273No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1260.15743309093635205No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT1210.1511857460579254No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG1090.13619211834970138No Hit
GCTGCGTTCTTCATCGATGCCAAATATACCGCTGTATCTATACTCCCCCC1090.13619211834970138No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG1070.13369318039833072No Hit
GCTGCGTTCTTCATCGATGCCAAATATACCGCCGTATCTATACTCCCCCC950.1186995526901067No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG880.10995326986030936No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG860.10745433190893869No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA860.10745433190893869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)