FastQCFastQC Report
Sun 21 May 2023
EGAF00007833223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88298
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4127446.74398061111237No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2252025.5045414392172No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG41374.685270334548914No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC39894.517656119051394No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC18512.0963102222020886No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGTTTTCAAAATAGGGT15751.7837323608688758No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC8020.9082878434392625No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7090.8029626945117669No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5550.6285533081156991No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT5210.5900473396905932No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA4930.5583365421640354No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTTAATAATGCCGTCATCGCCCATC4900.5549389567147613No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAATGATGTCGATCAAGAAGATTGCG4750.5379510294683911No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC4620.5232281591882035No Hit
GGAAGTAAAAGTCGTAACAAGGTAACTCGCGCGTCGTCTCAGCGCAAGTC3030.34315613037667897No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA3000.3397585449274049No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGAGCTGGCTCCCCTGCACACAATCA2680.3035176334684817No Hit
GGAAGTAAAAGTCGTAACAAGGGATGAAGCCGAAATGTTGCACCGCGCGG2110.2389635099322748No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGAGCGCGGAAGTG2030.22990328206754398No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTAGACGCAGCACGCGCAGTCGTA1900.21518041178735645No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1890.21404788330426508No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCCGCGCGTCGTCTCAGCGCAAGTC1850.2095177693718997No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC1770.2004575415071689No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT1760.19932501302407754No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT1640.18573467122698137No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA1590.18007202881152462No Hit
GGAAGTAAAAGTCGTAACAAGGTGTCGCAGATTTCCAATGCCTGTTCGCC1490.1687467439806111No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA1440.16308410156515438No Hit
GGAAGTAAAAGTCGTAACAAGGAGATCGAGAACAAGGAGGTTTTGCCATG1320.14949375976805818No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1270.1438311173526014No Hit
GGAAGTAAAAGTCGTAACAAGGAGCGTCGTATACTTACCGAAGAGGGGCT1140.12910824707241386No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1030.11665043375840903No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1030.11665043375840903No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG990.11212031982604362No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA980.11098779134295228No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC960.10872273437676958No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG930.10532514892749552No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC920.1041926204444042No Hit
GGAAGTAAAAGTCGTAACAAGGAGTACGTGAACGAGAAAACACTGCTTTA900.10192756347822146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)