FastQCFastQC Report
Sun 21 May 2023
EGAF00007833227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71629
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4691065.49023440226723No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA24293.3910846165659163No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT22623.157938823660808No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG22583.152354493291823No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA20492.8605732315123764No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC7231.0093677141939716No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT7221.0079716316017255No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG6650.9283949238436945No Hit
GGAAGTAAAAGTCGTAACAAGGCAATATGCGCCAAACCGCCCCTGCACAG5850.8167083164640021No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5490.7664493431431403No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC4580.63940582724874No Hit
GGAAGTAAAAGTCGTAACAAGGGTACGGGATGGGCTTACCCGCTCAGGCT4340.6058998450348322No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC4120.5751860280054168No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3570.4984014854318782No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC3570.4984014854318782No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC3180.44395426433427804No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2860.399279621382401No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGAATGAGGCTTTTGCCGAGTTTAA2600.3629814739840009No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG2400.33505982213907776No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2310.32249507880886236No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT2140.29876167474067766No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC1880.26246352734227757No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1800.25129486660430833No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1780.24850270141981598No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC1720.24012620586633904No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1690.23593795808960058No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC1530.21360063661366208No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1480.20662022365243127No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1430.1996398106912005No Hit
GGAAGTAAAAGTCGTAACAAGGCACTGGAAGGTTCAGGCTGCCCGCAATC1340.1870750673609851No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1310.1828868195842466No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGACAATCGCCGCAATCG1210.16892599366178504No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT1180.16473774588504655No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA1160.16194558070055423No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1150.1605494981083081No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAGGTTTTGGTGGGCAA1140.15915341551606194No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1050.1465886721858465No Hit
GGAAGTAAAAGTCGTAACAAGGGACTGGAGTTCGAAATCGGCTGGCGCGA1020.14240042440910805No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1010.1410043418168619No Hit
GGAAGTAAAAGTCGTAACAAGGTAACTTAGACGCAGCACGCGCAAGTCGT980.1368160940401234No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCTTAGACGCAGCACGCGCAAGTCGT980.1368160940401234No Hit
GGAAGTAAAAGTCGTAACAAGGTGTCCGTAGGTGAACCTGCGGAAGGATC960.1340239288556311No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC940.13123176367113878No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT930.12983568107889262No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG920.12843959848664646No Hit
GGAAGTAAAAGTCGTAACAAGGCAATGAAACAAGCATACAACAAAAAATG880.12285526811766183No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC860.12006310293316952No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC830.11587485515643105No Hit
GGAAGTAAAAGTCGTAACAAGGCCATCGCCATGGAAGGATTAAAAAACAA800.11168660737969258No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC800.11168660737969258No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC760.10610227701070794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)