FastQCFastQC Report
Sun 21 May 2023
EGAF00007833230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145333
Sequences flagged as poor quality0
Sequence length236-238
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3784026.03675696503891No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT2915820.06289005250012No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2654318.263573999022935No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACAATGTGTT72494.987855476732745No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG54603.756889350663648No Hit
GCTGCGTTCTTCATCGATGCCAGTGAGATACGGCTGCGTTCTTCATCGAT23051.5860128119559909No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT22821.5701870875850632No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT18191.251608375248567No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA14751.0149105846573043No Hit
GCTGCGTTCTTCATCGATGCAGTGAGATACGGCTGCGTTCTTCATCGATG14090.9694976364624689No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT12720.8752313652095531No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT12500.8600937158112748No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG12190.8387633916591551No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT7820.5380746286115335No Hit
GCTGCGTTCTTCATCGATGCTGTCCGCGTAATCGTGGATTGTCTTATGAC7790.5360104036935864No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA7720.5311938788850433No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC7670.5277535040217982No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7580.5215608292679571No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT7190.4947259053346453No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT6370.4383037575774257No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC5580.3839458347381531No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT4950.3405971114612648No Hit
GCTGCGTTCTTCATCGATGCACCTTTCAATCTTAAATACTACAAAAAGGG4090.2814226638134491No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGGT3840.2642207894972236No Hit
GCTGCGTTCTTCATCGATGCGCAAGGATAAGAACACGCTTACCCCTGCGA3820.2628446395519256No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG3760.25871618971603144No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT3410.23463356567331575No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3300.22706474097417656No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCTGGCAAACT3200.22018399124768634No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT3090.21261516654854712No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT3060.21055094163060006No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG3030.20848671671265304No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC3030.20848671671265304No Hit
GCTGCGTTCTTCATCGATGCCAGTGAGATACGGCTGCGTTCTTCATCGGC2910.20022981704086476No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATCATGGC2730.18784446753318243No Hit
GCTGCGTTCTTCATCGATGCTGATTTCGCCGTTGTGAATACTGCCGCTAA2680.18440409266993732No Hit
GCTGCGTTCTTCATCGATGCTTGCCCAGTGCATCTGACCACTGACGAACA2620.18027564283404318No Hit
GCTGCGTTCTTCATCGATGCGGCATACAAGGCCTGATGCCATGGGAAGAC2330.16032146862722163No Hit
GCTGCGTTCTTCATCGATTTCCAGGATCATGACTTCAACTTCGTCACCCA2270.1561930187913275No Hit
GCTGCGTTCTTCATCGATGCTGTCCGCGTAATCGTAGATTGTCTTATGAC2190.15068841901013535No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG2080.14311959431099613No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1960.1348626946392079No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT1960.1348626946392079No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG1930.13279846972126083No Hit
GCTGCGTTCTTCATCGATGCGCTGCTGCGTCTCGGTCTTTGCGGCCGGAC1930.13279846972126083No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1890.13004616983066475No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAGGTTACCTTGTTACGA1820.1252296450221216No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1790.12316542010417456No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT1780.12247734513152553No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1730.11903697026828042No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGTCGAGAGTCG1680.11559659540503534No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACTATCGGGCAAACT1680.11559659540503534No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1670.11490852043238632No Hit
GCTGCGTTCTTCATCGATGCAGTGAGATACGGCTGCGTTCTTCATCGGCT1660.1142204454597373No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1600.11009199562384317No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT1530.10527547081530003No Hit
GCTGCGTTCTTCATCGATGCGGCATACAAGGCCTGATGCCATGGGGAGGC1520.10458739584265102No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT1510.10389932087000199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)