FastQCFastQC Report
Sun 21 May 2023
EGAF00007833239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103096
Sequences flagged as poor quality0
Sequence length247-250
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC7261870.43726235741445No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1263512.255567626290059No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA12351.197912625126096No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC11861.15038410801583No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG10170.9864592224722588No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC10120.9816093737875379No Hit
GGAAGTAAAAGTCGTAACAAGGTCGGGGCGGGCACGGGCGGGAGGGGCTC9780.9486304027314348No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC6060.5878016605881896No Hit
GGAAGTAAAAGTCGTAACAAGGCTATCAACTGGACGGCTTACGTCAACAT4120.3996275316210134No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCAGACCTTGG3500.3394894079304726No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTACCGACCTCACCGTTGATGA3350.32493986187630947No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG3310.32105998292853266No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3270.3171801039807558No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3210.31136028555909057No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3130.3036005276635369No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC2920.2832311631877085No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2860.2774113447660433No Hit
GGAAGTAAAAGTCGTAACAAGGCCTCACCTGACACCATGGAGTACTCTGC2450.2376425855513308No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC2290.2221230697602235No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2250.21824319081244664No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2150.20854349344300457No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1870.18138434080856677No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1860.18041437107162256No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC1730.16780476449134787No Hit
GGAAGTAAAAGTCGTAACAAGGCATCATCGAACATTTTGAATATGTGGCC1500.14549546054163112No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1450.14064561185691007No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1410.13676573290913324No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1390.1348257934352448No Hit
GGAAGTAAAAGTCGTAACAAGGGGCTCGGGAAAAAGGCAAAGAGAAACAT1330.12900597501357958No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1170.11348645922247226No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1150.11154651974858384No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1080.1047567315899744No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1070.1037867618530302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)