FastQCFastQC Report
Sun 21 May 2023
EGAF00007833243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76149
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5027066.01531208551656No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC38285.0269865658117645No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC24293.1897989468016656No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT13371.7557682963663346No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC12891.6927339820614846No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG12431.632326097519337No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT11581.5207028326044991No Hit
GGAAGTAAAAGTCGTAACAAGGTGCTTGTTGCCCTGTTGTGAACTCACGT8061.0584511943689348No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG7140.9376354252846393No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC3920.5147802334896059No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC3630.47669700193042586No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3410.4478062745407031No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC2790.3663869518969389No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT2560.3361830096258651No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA2420.31779800128695057No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2370.3112319268801954No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC2330.30597906735479125No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC2150.28234119949047265No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC2130.27971476972777054No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2050.26920905067696227No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTAAGCAGAAAACAAAACAAACAACG1990.26132976138885605No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC1970.258703331626154No Hit
GGAAGTAAAAGTCGTAACAAGGAGTAAAACGAGCTGGTTCCGATTATAGA1850.24294475304994156No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1790.23506546376183532No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1760.23112581911778224No Hit
GGAAGTAAAAGTCGTAACAAGACCAGATGACGACGCCATAGGCGACGAGC1700.22324652982967605No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC1570.2061747363721126No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1570.2061747363721126No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAGAAGATGAAGCACATATTGTTTG1410.185163298270496No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1360.17859722386374083No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA1330.17465757921968772No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCGCAGACACGTGGAACGCACGA1270.1667782899315815No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1210.15889900064347529No Hit
GGAAGTAAAAGTCGTAACAAGGGAGTGCTCTCAGAAGAAACTGGCGAGGG1190.15627257088077323No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1170.15364614111807115No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG1160.15233292623672012No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACCTGCACATAGAAACA1120.147080066711316No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAGGAAAGCCGTATCAGACCGAATA1110.14576685182996493No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1110.14576685182996493No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1100.1444536369486139No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1010.13263470301645458No Hit
GGAAGTAAAAGTCGTAACAAGGATATATTCTGCTGTATAGAAGCAAGGTT1000.13132148813510353No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC980.12869505837240147No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTGGGGCAGAAGGTGGAAAATGCGCG960.1260686286096994No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAAACTGCAGAAGGATC920.12081576908429525No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTCCGGAAGGATC890.11687612444024216No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGTATGAAACTCACCGAAAGCTAC880.11556290955889112No Hit
GGAAGTAAAAGTCGTAACAAGGTCATGAACGGTATCACCGACTGGGTCTA820.1076836202707849No Hit
GGAAGTAAAAGTCGTAACAAGGCATTGATGGTGTCGGTGAGGTTGTGCGG780.10243076074538077No Hit
GGAAGTAAAAGTCGTAACAAGGACGTAAATAATCAATTAAAATCACAGTA770.10111754586402974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)