FastQCFastQC Report
Sun 21 May 2023
EGAF00007833244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76149
Sequences flagged as poor quality0
Sequence length235-238
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2388931.371390300594886No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2186828.717383025384446No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG37584.935061524117192No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG32754.300778736424641No Hit
GCTGCGTTCTTCATCGATTTCGCCCAACAATGCTTCAGGAGAAACGACAA24363.1989914509711226No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC12901.694047196942836No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT12431.632326097519337No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG12411.629699667756635No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT9611.2619995009783451No Hit
GCTGCGTTCTTCATCGATCAAGTGGAGTTTAAAGCAAAGACCAGGGTTTT8211.0781494175892001No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT7150.9389486401659904No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3480.45699877871016037No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG3450.45305913406610726No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2260.29678656318533403No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT2240.294160133422632No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA2150.28234119949047265No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTTGCACATCTGCG2030.2665826209142602No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1980.26001654650750505No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1970.258703331626154No Hit
GCTGCGTTCTTCATCGATGCAAACTATGCGGAAGCTCTTGAATATGTCGA1900.24951082745669675No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1840.2416315381685905No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG1760.23112581911778224No Hit
GCTGCGTTCTTCATCGAATCACTGGACAACATCATCGGTCAGCTCAATAC1760.23112581911778224No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG1730.22718617447372913No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1730.22718617447372913No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1610.21142759589751672No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG1560.20486152149076153No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT1490.1956690173213043No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG1480.19435580243995326No Hit
GCTGCGTTCTTCATCGATTCTCCATGCCGTTATATGGATTTCATAACTAA1470.19304258755860224No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG1460.19172937267725118No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1450.19041615779590015No Hit
GCTGCGTTCTTCATCGATGTGCCTGCCACGGCTACGATGTTGTAGCCCCG1450.19041615779590015No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1400.18385008338914496No Hit
GCTGCGTTCTTCATCGATGCCTCATCCTTCAGCTTCGAGAAGATACCCTC1380.1812236536264429No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT1330.17465757921968772No Hit
GCTGCGTTCTTCATCGATGTGCAGAATTTGGGGCCAGAGTTGCAATATCT1240.1628386452875284No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGAGATCCGTTGTTGAAAGTTT1210.15889900064347529No Hit
GCTGCGTTCTTCATCGATGCCTAGATGGTCACCACCCCCACTCCCTGGGG1190.15627257088077323No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1160.15233292623672012No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT1150.15101971135536907No Hit
GCTGCGTTCTTCATCGACACCTTGCCGTATATGTCTATGAAGGCGGCAGA1110.14576685182996493No Hit
GCTGCGTTCTTCATCGATTTAGGTACGTTGGTGAAACTGTATGGTATCGT1020.1339479178978056No Hit
GCTGCGTTCTTCATCGATGCCGGTAACGTGTGGCTCCTTCGTCCCGATGC940.12344219884699732No Hit
GCTGCGTTCTTCATCGATGCGAGAGACAAGAGATCCGTTGTTGAAAGTTG920.12081576908429525No Hit
GCTGCGTTCTTCATCGATGCGACCTAAAGTGAAATAGTTGCTTGCAGGGT910.11950255420294423No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT860.11293647979618905No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT860.11293647979618905No Hit
GCTGCGTTCTTCATCGATGCGGGGGATGTAGTAGGGTGCGCTCTTGAAGT810.10637040538943388No Hit
GCTGCGTTCTTCATCGACGGTCAGGGCAACTTCGGTTCGGTTGACGGCGA790.10374397562673181No Hit
GCTGCGTTCTTCATCGATGCCAGCCACAAAACCTGCGGCAACAGTTGTGT770.10111754586402974No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG770.10111754586402974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)