FastQCFastQC Report
Sun 21 May 2023
EGAF00007833246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108835
Sequences flagged as poor quality0
Sequence length236-238
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG8104774.46777231589103No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT98239.025589194652456No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG21501.9754674507281664No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT14581.3396425782147288No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG13011.1953875132080671No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10310.9473055542794138No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT6340.5825331924472826No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT5920.5439426655028253No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5040.4630863233334864No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4940.4538981026324252No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGAT4930.45297928056231906No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGATG4830.44379105986125783No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG4780.4391969495107273No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG2730.2508384251389718No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG2700.24808195892865345No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA2680.24624431478844122No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCTGAGAGTCG2530.23246198373684934No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT2250.20673496577387787No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAAGTCA2220.2039784995635595No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2060.18927734644186153No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG1960.1800891257408003No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1940.17825148160058804No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1830.16814443882942068No Hit
GCTGCGTTCTTCATCGATGCGAGAGACGAGATATCCGTTGCCGAGAGTCG1770.16263150640878393No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT1570.14425506500666146No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG1450.13322920016538797No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1420.13047273395506961No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1410.12955391188496349No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1400.12863508981485736No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT1390.12771626774475123No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG1250.11485275876326549No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG1140.10474571599209813No Hit
GCTGCGTTCTTCATCGATGCCATAACCAAGAGATCCGTTGTTGAAAGTTT1140.10474571599209813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)