FastQCFastQC Report
Sun 21 May 2023
EGAF00007833253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187497
Sequences flagged as poor quality0
Sequence length247-250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC11444161.03617657882526No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC120876.446503144050304No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGAATGAGGCTTTTGCCGAGTTTAA54222.891779601806962No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT48312.57657455852627No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC43892.340837453399254No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG26311.4032224515592249No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG21501.1466850136268847No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG20691.1034843224158253No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG19111.0192163074609195No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTCCCGACCTCGCCGTTGATGA17350.9253481389035558No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC16860.899214387430199No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT14890.7941460396699681No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC11020.5877427372171288No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC9130.4869411243913236No Hit
GGAAGTAAAAGTCGTAACAAGGAAGCAATGTTACTACACGTGGCACTACT6920.3690725718278159No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC6700.3573390507581455No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC5780.3082715990122509No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC5210.27787111260446834No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA5030.2682709590020107No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4820.25707077979914345No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA4790.25547075419873383No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT4610.24587060059627622No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC4550.24267054939545699No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA4030.21493677232169048No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG3830.20426993498562646No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC3580.19093638831554638No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA3400.18133623471308874No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC3300.17600281604505671No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGGCACGTCAGTATATGTGCGAGCA3260.17386944857784392No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC3150.1680026880430087No Hit
GGAAGTAAAAGTCGTAACAAGCACACAATGAACTTACTAATAGTTCAATC3120.16640266244259908No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC3040.16213592750817346No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAGGTTTTGGTGGGCAA3030.16160258564137026No Hit
GGAAGTAAAAGTCGTAACAAGGTGGTGGAGGCCATCAACAACGGCTTCCT3000.16000256004096064No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA2890.15413579950612544No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT2860.15253577390571582No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2610.13920222723563577No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC2550.13600217603481657No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT2460.13120209923358775No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTACCGACCTCACCGTTGATGA2440.13013541549998134No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC2380.12693536429916213No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2300.12266862936473649No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC2280.12160194563113011No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2270.1210686037643269No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG2140.11413515949588526No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2060.10986842456145965No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC2060.10986842456145965No Hit
GGAAGTAAAAGTCGTAACATGAGTGCAGGCTTCCAGGAACTGGCAACAGG2010.10720171522744364No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC2000.10666837336064043No Hit
GGAAGTAAAAGTCGTAACAAGAAGACCATCAAGGCCCTCTGCGACAGACC1880.10026827095900202No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)