FastQCFastQC Report
Sun 21 May 2023
EGAF00007833255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86858
Sequences flagged as poor quality0
Sequence length247-250
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5750866.20921504064104No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG41694.799788159985264No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA39384.533836837136476No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA32263.7141080844596925No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC24032.766584540284142No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT19672.2646158097124043No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTTGTGCG12341.420709664049368No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG9121.0499896382601488No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4890.5629878652513297No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4080.46973220659006654No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3240.37302263464505286No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT3120.3592069815100509No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC2970.34193741509129844No Hit
GGAAGTAAAAGTCGTAACAAGGAGATGCAGAAAACGGCAGCGCTTCGGGA2780.32006263096087867No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2700.310852195537544No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGATTTTGCGTGAAGGCATGAACGTC2350.27055654056045497No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2340.2694052361325382No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2340.2694052361325382No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC2310.26595132284878764No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2300.26480001842087086No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT1990.2291095811554491No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTAAACTGAATCCGAAAAGAGCACA1740.20032697045752837No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1730.19917566602961154No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1720.1980243616016947No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA1510.1738469686154413No Hit
GGAAGTAAAAGTCGTAACAAGGTATCGCAGATTTCCAATGCCTGTTCGCC1500.17269566418752447No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1470.16924175090377397No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1180.13585392249418593No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1140.1312487047825186No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT1120.12894609592668493No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1100.12664348707085127No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1070.12318957378710078No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTTAATAATGCCGTCATCGCCCATC1000.11513044279168297No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA960.11052522508001567No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA960.11052522508001567No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC920.10592000736834833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)