FastQCFastQC Report
Sun 21 May 2023
EGAF00007833270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135326
Sequences flagged as poor quality0
Sequence length235-238
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2888321.343274758730768No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2172416.053086620457265No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT1800813.307125016626516No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG1464510.822014986033727No Hit
GCTGCGTTCTTCATCGATGCAGGCAAGCACTGGCTGCGTTCTTCATCGAT70565.214075639566676No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG56434.169930390316717No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT50303.7169501795663806No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT33102.445945346792191No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT18641.377414539704122No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT16621.228145367482967No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC13801.0197596914118499No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT12890.9525146682825179No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG12500.923695372655661No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT9630.7116149150939214No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG9220.6813177068708156No Hit
GCTGCGTTCTTCATCGATGCAGGCAAGCACTGGCTGCGTTCTTCATCGGC8450.6244180719152269No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT8430.6229401593189778No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT7360.5438718354196532No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6370.4707151619053249No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG6370.4707151619053249No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT6330.46775933671282677No Hit
GCTGCGTTCTTCATCGATGCTGGAGCCAAGAGATCCGTTGCTGAAAGTTT5240.3872131002172532No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5170.3820404061303814No Hit
GCTGCGTTCTTCATCGATGAGGCAAGCACTGGCTGCGTTCTTCATCGATG5070.37465084314913616No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT4810.3554379793978984No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG3900.2881929562685663No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG3800.28080339328732096No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG3600.2660242673248304No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT3590.2652853110267059No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3520.2601126169398342No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3340.24681140357359269No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA3160.23351019020735114No Hit
GCTGCGTTCTTCATCGATGCAGGCAAGCACTGGCTGCGTTCTTCATCGCC3020.22316480203360772No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC2900.21429732645611338No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA2860.21134150126361526No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2830.20912463236924167No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTGAAAGTTG2760.20395193828237No Hit
GCTGCGTTCTTCATCGATGCAGACCTGTGTCTACCCTCGTGTGACACTGG2750.20321298198424545No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG2670.19730133159924923No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT2550.1884338560217549No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA2260.16700412337614354No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT2040.1507470848174039No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG2010.1485302159230303No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGTACGCTCGGCGC1870.13818482774928692No Hit
GCTGCGTTCTTCATCGATGGGAGAACAAAGAGATCCGTTGTCGAAAGTTG1870.13818482774928692No Hit
GCTGCGTTCTTCATCGATGCACCTGAGCCAATTTTTGGCTTGGCGGTAAC1760.1300563084699171No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT1640.12118883289242274No Hit
GCTGCGTTCTTCATCGATGCGGCAAGCACTGGCTGCGTTCTTCATCGATG1510.11158240101680386No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAATCGTACTCCACAACAGG1480.10936553212243028No Hit
GCTGCGTTCTTCATCGATGCGTCCAGCGCCGATCCCGAAGCGAAGTTGGA1440.10640970692993217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)