FastQCFastQC Report
Sun 21 May 2023
EGAF00007833271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407121
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA19735148.474777768771446No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC6747516.573696763370105No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC5150012.649801901645949No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA183604.509715784742128No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG102462.5166965103740657No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC46771.1487985144465651No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA43491.0682327858302567No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC33890.8324306532947208No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC26480.6504208822438539No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC25140.6175068345774353No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC23160.5688726447419808No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT21350.5244141176701766No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT17600.43230390964848286No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG17540.4308301463201358No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT16020.3934948086686759No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA14180.34829939993269815No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA11560.28394506792820806No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA10380.25496105580404843No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA10130.24882037526926887No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA9590.2355565053141449No Hit
GGAAGTAAAAGTCGTAACAAGGCGCGGCGAAGCTCGTCTTCACCAAGCCT8920.21909948148093567No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC8440.20730937485415885No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA8310.20411622097607343No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7230.17758848106582564No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGATGAAGGACACCTCTCCGGCA6870.16874590109574303No Hit
GGAAGTAAAAGTCGTAACACGCCATACGGGTATTCGTGTTTTCGGAATGT6340.15572765836201033No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC6120.15032385949140428No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6080.14934135060583953No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCAGGCA5120.12576113735228595No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTATCCGGCA5070.12453300124533001No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGAGCGGAAGTG5060.12428737402393883No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA4800.11790106626776806No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA4490.11028662240464136No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG4430.10881285907629427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)