FastQCFastQC Report
Sun 21 May 2023
EGAF00007833272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407121
Sequences flagged as poor quality0
Sequence length235-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT18697845.9268866012807No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG5066712.445194426227092No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4398710.804404587333986No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT183264.501364459214828No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG171134.203418639667323No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG95092.3356692482087635No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT75051.8434322965408318No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT45441.1161300940015377No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT35450.8707484998317453No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT34310.8427469965931504No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT31330.7695500846185778No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG26170.6428064383807272No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT20940.5143434015931382No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAGGTCA20390.5008339044166231No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT18300.44949781514586573No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC17510.4300932646559622No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG17050.4187944124719678No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT16230.39865298031789076No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCTGGCAAACT14500.356159471017216No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGTCAAACT12950.3180872517015826No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT9700.23825840474944793No Hit
GCTGCGTTCTTCATCGATGCGGTCACGACGGAATTCCCGGCGGGCGGCAT8820.2166432092670238No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG8420.20681812041137645No Hit
GCTGCGTTCTTCATCGATGCCATGAATCCCTGCAGATATCCAACCAGCTC8280.20337933931189991No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG7420.18225539827225812No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA7370.1810272621653022No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACTATCGGGCAAACT6920.16997403720269894No Hit
TCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT6480.15916643946148687No Hit
GCTGCGTTCTTCATCGATGCAAGGGATGAAGCTCTCTTTTTGTCTCTTTC5870.1441831789566247No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC5840.14344629729245115No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG5340.13116493622289196No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT5240.12870866400898012No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG5240.12870866400898012No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG5220.12821740956619776No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG5170.12698927345924185No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGACAAACT5070.12453300124533001No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC5060.12428737402393883No Hit
GCTGCGTTCTTCATCGATGTCGTTTCCGAGGCCGGACGATCGGGCAAACT4700.11544479405385623No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAATCGTACTCCACAACAGG4530.11126913129020612No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGTCCGGACGATCGGGCAAACT4240.10414594186986179No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT4180.1026721785415147No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT4160.10218092409873232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)