FastQCFastQC Report
Sun 21 May 2023
EGAF00007833274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100373
Sequences flagged as poor quality0
Sequence length235-238
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT3823638.0939097167565No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3384733.72121984995965No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1152111.478186364859075No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT23942.3851035637073714No Hit
GCTGCGTTCTTCATCGATGCTGTCCGATGTTACGGTAGTGGGCGTCTTCA13981.39280483795443No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT8330.8299044563777112No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5590.5569226784095325No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT5180.5160750401004255No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT4550.45330915684496825No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG4340.4323871957598159No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3880.3865581381447202No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2800.27895948113536506No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT2560.2550486684666195No Hit
GCTGCGTTCTTCATCGATGCGCAAGGATAAGAACACGCTTACCCCTGCGA2330.23213413965907168No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2320.23113785579787394No Hit
GCTGCGTTCTTCATCGATGCCGGTAACGTGTGGCTCCTTCGTCCCGATGA1990.19826048837834875No Hit
GCTGCGTTCTTCATCGATGCCATGAATCCCTGCAGATATCCAACCAGCTC1820.1813236627379873No Hit
TCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1720.17136082412601No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGATGCCGGACGATCGGGCAAACT1420.141472308290078No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT1390.13848345670648482No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAATACTGTAATGTCTTC1320.13150946967810068No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1210.12055034720492563No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1160.11556892789893697No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1100.10959122473175058No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC1070.10660237314815738No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCTGACGATCGGGCAAACT1060.10560608928695964No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1040.10361352156456419No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG1030.10261723770336643No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)