FastQCFastQC Report
Sun 21 May 2023
EGAF00007833283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80570
Sequences flagged as poor quality0
Sequence length247-250
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4838960.05833436763063No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG1191114.783418145711805No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC37304.6295147077075836No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC28023.4777212361921306No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG15671.9448926399404245No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA12441.5439990070745935No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC11891.4757353853791735No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC6030.7484175251334243No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG4870.6044433411939928No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT4730.5870671465806132No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC4380.543626660047164No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG4200.5212858384013901No Hit
GGAAGTAAAAGTCGTAACAAGGAACGATGAGTGCCAAGAAGGAAAAAAAG3200.39717016259153526No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2940.3649000868809731No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA2250.2792602705721733No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1990.24699019486161103No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCT1950.2420255678292168No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGGGTGATGTCCGCGGAGATGAGCT1830.22713168673203424No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1630.20230855157006328No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1200.14893881097182574No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1190.1476976542137272No Hit
GGAAGTAAAAGTCGTAACAAGGTATCTGTAGGTGAACCTGCAGATGGATC1130.14025071366513592No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACACATGGCTCAAGCAGAGCAAAA1070.13280377311654462No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGCTGGCATCTCAACAATGACGG1030.12783914608415042No Hit
GGAAGTAAAAGTCGTAACAAGGCCAAAGAAGAAGAGGAAAAGAAGGCGGC1020.12659798932605187No Hit
GGAAGTAAAAGTCGTAACAAGGAGGTGCGTGAGATCGTGCAGCAGAGCCT980.12163336229365769No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA920.11418642174506641No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC920.11418642174506641No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGACAGAGGGAGTAGCCTGAAAAC900.11170410822886931No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGTGGGTGAGAGGGAAGGGGCCACC880.10922179471267221No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)