FastQCFastQC Report
Sun 21 May 2023
EGAF00007833301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143366
Sequences flagged as poor quality0
Sequence length247-250
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC11596580.88737915544829No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA79985.5787285688378No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC24631.717980553269255No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC15011.0469706904007925No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG14891.0386005050011857No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA8170.5698701226232161No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6770.4722179596278057No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC5040.35154778678347726No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC4080.28458630358662446No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCGTAAGTTAATAC3870.2699384791373129No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT3700.2580807164878702No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC3490.24343289203855867No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC3320.23157512938911598No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC3290.2294825830392143No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC3100.2162297894898372No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCTTAGACGCAGCACGCGCAAGTCGT3070.21413724313993554No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTAGACGCAGCACGCGCAAGTCGT3010.20995215044013224No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC2920.2036745113904273No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC2350.16391613074229594No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2350.16391613074229594No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT2130.14857079084301716No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC2060.14368818269324665No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2050.14299066724327944No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTAGACGCAGCACGCGCAGTCGTA1960.1367130281935745No Hit
GGAAGTAAAAGTCGTAACAAGGTAACTTAGACGCAGCACGCGCAAGTCGT1750.12206520374426293No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1720.11997265739436128No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAGTAAAAACCCTGAAA1690.11788011104445964No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1650.11509004924459076No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1590.11090495654478746No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1560.10881241019488581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)