FastQCFastQC Report
Sun 21 May 2023
EGAF00007833312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158110
Sequences flagged as poor quality0
Sequence length235-238
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3796324.01049901966985No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2385415.08696477136171No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG130658.263234457023591No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT99576.297514388716715No Hit
GCTGCGTTCTTCATCGATGCGCAAGGATAAGAACACGCTTACCCCTGCGA82605.2242109923471No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG80395.084434887103915No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT72204.5664410853203465No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA57943.664537347416356No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG57393.6297514388716716No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG38412.4293213585478464No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG24051.5210929099993675No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG20841.3180696983113023No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG20231.2794889633799253No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT13540.8563658212636771No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT13140.8310669786857251No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC13030.8241097969767883No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10320.6527101385111631No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT10010.6331035355132503No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT6350.40161912592498894No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6200.3921320599582569No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5670.35861109354247045No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG5560.3516539118335336No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT5470.34596167225349445No Hit
GCTGCGTTCTTCATCGATGCCCTTTCCTCCGCCTCCCTTACGCCTGGGCC4720.2985263424198343No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA4250.2688002023907406No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG4230.26753526026184304No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG4030.254885838972867No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC3940.24919359939282776No Hit
GCTGCGTTCTTCATCGATGCACGATCCTTCCTCGCTGCAGGATCCTTCCT3700.23401429384605651No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC3610.22832205426601732No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3600.2276895832015685No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC3490.22073240149263174No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGTTGAAAGTTG3460.2188349882992853No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGCTGAAAGTTG3340.2112453355258997No Hit
GCTGCGTTCTTCATCGATGAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT3300.20871545126810448No Hit
GCTGCGTTCTTCATCGATGCGGTCGCGGTCGGCGCAGTCCACTACGAAGA3090.19543355891467967No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT3060.19353614572133326No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTCCACTTCGCAAATCAAAATAA2700.1707671874011764No Hit
GCTGCGTTCTTCATCGATGCCTTTCGGCGTTATCCGTATGTTGTTCACCC2600.16444247675668838No Hit
GCTGCGTTCTTCATCGATGCACGATCATTCCTGGTTGCACGATCCTTCCT2510.15875023717664916No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT2320.14673328695212193No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACATCCAAGTAGCAG2170.1372462209853899No Hit
GCTGCGTTCTTCATCGATGCGGTCCGCCATCGCCCCGTCGGAGCCGACGA2120.1340838656631459No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT2090.1321864524697995No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG1930.12206691543861867No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1880.11890456011637467No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACTTGG1860.11763961798747707No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA1810.11447726266523306No Hit
GCTGCGTTCTTCATCGATGCCCACCCGGACCCGCACGCCATCGCCGAGTT1710.10815255202074506No Hit
GCTGCGTTCTTCATCGATGCGTACGTGACGGCGGAGCCTACGGCCCCGGC1680.10625513882739865No Hit
GCTGCGTTCTTCATCGATGCTTTCCTAAGGACCCCGGGCACCTTCTCCAG1680.10625513882739865No Hit
GCTGCGTTCTTCATCGATGCGGTGTCCGAGGCGTGGCATTTCACCTCCAC1640.10372525456960344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)