FastQCFastQC Report
Sun 21 May 2023
EGAF00007833321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84459
Sequences flagged as poor quality0
Sequence length247-250
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC6348875.17020092589304No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA74648.83742407558697No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC28143.33179412496004No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG8040.9519411785600113No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7130.8441965924294628No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT4930.5837151754105543No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3730.44163440249114955No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG3190.37769805467741746No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG2980.3528339194165216No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG2800.33152180347861093No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2380.2817935329568193No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC2320.27468949431084905No Hit
GGAAGTAAAAGTCGTAACAAGGTGTCGCAGATTTCCAATGCCTGTTCGCC2280.2699534685468689No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2240.2652174427828888No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG1970.2332492688760227No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1670.19772907564617154No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAAGTGAACCTGCGGAAGGATC1450.17168093394428066No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1390.16457689529831043No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1350.15984086953433027No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1340.1586568630933352No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT1320.15628885021134514No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1320.15628885021134514No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1300.15392083732935505No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1290.15273683088836004No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1120.1326087213914444No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1080.12787269562746423No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1000.11840064409950389No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC960.11366461833552376No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC950.11248061189452871No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC940.11129660545353368No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC920.1089285925715436No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC850.1006405474845783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)