FastQCFastQC Report
Sun 21 May 2023
EGAF00007833340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73626
Sequences flagged as poor quality0
Sequence length235-238
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2734837.14448700187434No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2720536.950262135658605No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG33174.505201966696547No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT15322.080786678618966No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT13381.817292804172439No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT12361.6787547877108628No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT11001.4940374324287615No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT9921.3473501208812104No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT8771.1911552984000218No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5940.8067802135115312No Hit
GCTGCGTTCTTCATCGATGCAGGTATCGAGCATGCCCTCAAGGCCTTCGA4820.6546600385733301No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3470.4713008991388911No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA2970.4033901067557656No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2640.3585689837829028No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT2250.3055985657240649No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG2190.29744927063808985No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG1930.2621356585988645No Hit
TCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1550.21052345638768916No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG1300.17656806019612636No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1300.17656806019612636No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1290.17520984434846387No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA1080.14668731154755113No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT940.127672289680276No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT930.12631407383261348No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG930.12631407383261348No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCTAGAGTCG900.12223942628962596No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG880.11952299459430092No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG840.11409013120365087No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC800.10865726781300084No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATACGTTGTTGAAAGTTT790.10729905196533833No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT760.1032244044223508No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG760.1032244044223508No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGATATCCGTTGCCGAGAGTCG750.1018661885746883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)