FastQCFastQC Report
Sun 21 May 2023
EGAF00007833363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75326
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2963539.34232535910575No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC866611.504659745638957No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC809410.745293789660941No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC74699.915567002097548No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG57577.642779385603908No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG37224.941188965297507No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA9961.3222526086610202No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC9951.3209250458009187No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG4940.6558160528901044No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA4450.5907654727451345No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT4330.5748347184239173No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC3950.5243873297400632No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2450.3252529007248493No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2410.3199426492844436No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2350.3119772721238351No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG2320.30799458354353076No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAGTCGTA2150.2854260149218065No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGGGACGGCGGAAAATG2090.277460637761198No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAGGAAAGAAAAGGAGCACACATGAA1730.22966837479754665No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1680.22303056049703956No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1340.17789342325359106No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1320.1752382975333882No Hit
GGAAGTAAAAGTCGTAACAAGGACCTTCAAAAGGGGAGCGCGCGGAAGTG1310.17391073467328677No Hit
GGAAGTAAAAGTCGTAACAAGGATGGAAAGAGGTGGGGGAGGAGAAGAGG1100.14603191461115683No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCTTAGACGCAGCACGCGCAGTCGTA1030.13673897459044684No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGAGAGAGAGATGGGGAAGGGGACAG970.1287735974298383No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT960.12744603456973688No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGAGGGCAAGGAAAAGTGGAGGAG890.11815309454902691No Hit
GGAAGTAAAAGTCGTAACAAGGGAACAGAAAGGGCATAAATAAGAAAATA810.1075325916682155No Hit
GGAAGTAAAAGTCGTAACAAGCCAAAACCTGTGACATCAGTCATCGCTTT800.10620502880811407No Hit
GGAAGTAAAAGTCGTAACAAGGTACGGACGCATCGATGAAGAACGCAGCC770.10222234022780978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)