FastQCFastQC Report
Sun 21 May 2023
EGAF00007833364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75326
Sequences flagged as poor quality0
Sequence length236-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1107114.697448424182886No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT947012.572020285160502No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT884611.743621060457214No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA766310.173114196957226No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT61848.209648726867218No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG60047.97068741204896No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT36274.815070493587871No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT32564.322544672490243No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG16452.1838409048668455No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAACACTGTAATGTCTTC10061.3355282372620343No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9911.315614794360513No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT8261.0965669224437777No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT8041.0673605395215464No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG6760.8974324934285639No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT5170.6863499986724372No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC4190.5562488383824974No Hit
GCTGCGTTCTTCATCGCAGCTTCGACAGTGAAGCAGTTCAGGTCGACAAT4040.536335395480976No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG3930.5217322040198603No Hit
GCTGCGTTCTTCATCGATGCCAAATATACCGCCGTGTCTATACTCCCCCC3740.4965085096779332No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT3270.43411305525316624No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGCTGAAAGTTT3150.4181823009319492No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT2750.3650797865278921No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTAAAAGTTT2720.3610970979475878No Hit
GCTGCGTTCTTCATCGATGCGTCCGTACCTTGGCTGCGTTCTTCATCGAT2710.3597695350874864No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG2470.3279080264450522No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT2350.3119772721238351No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG2320.30799458354353076No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT2210.2933913920824151No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1980.26285744630008234No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1810.24028887767835808No Hit
GCTGCGTTCTTCATCGATGCGGGGATATCCAGAATCGCCCAATGCCACCA1710.22701324907734383No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT1570.20842736903592382No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1500.1991344290152139No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1500.1991344290152139No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG1370.18187611183389532No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1360.1805485489737939No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1300.17258317181318536No Hit
GCTGCGTTCTTCATCGATGCCCGGCTTCCTTTTTCTCTTTATCGAAAAAA1190.15797998035206967No Hit
GCTGCGTTCTTCATCGATGCTGCACAGAGCCTGCATCCTCCTCCTCCTCG1130.15001460319146112No Hit
GCTGCGTTCTTCATCGATGCCGGTGAATTATGCGATGGTGGTGGCGGCGG1060.14072166317075113No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTTTTAAAAGTTT1040.13806653745054828No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1020.13541141173034543No Hit
GCTGCGTTCTTCATCGATGCCAACAGCGTTAGGTGTCCCTCCCAGGTTCC1020.13541141173034543No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG990.13142872315004117No Hit
GCTGCGTTCTTCATCGATGCGTCCGTACCTTGGCTGCGTTCTTCATCGGC980.13010116028993973No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT970.1287735974298383No Hit
GCTGCGTTCTTCATCGATGCACCTGAGCCAATTTTTGGCTTGGCGGTAAC820.1088601545283169No Hit
GCTGCGTTCTTCATCGATGCCGCCATGGTAGGTAAACTGGGAGCAGAAGC820.1088601545283169No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT800.10620502880811407No Hit
GCTGCGTTCTTCATCGACACGCCCTTCTTTCTCTCTCTCTCTCTCTCTCT790.10487746594801264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)