FastQCFastQC Report
Sun 21 May 2023
EGAF00007833377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110835
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5255747.41913655433753No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG1268411.444038435512248No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT51764.670004962331394No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC42163.803852573645509No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC31972.8844679027383044No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC29932.7004105201425546No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC28352.5578562728380025No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC20101.8135065638110706No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC18671.6844859475797356No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC16941.5283980692019667No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA14771.3326115396760951No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC12531.1305093156493888No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG10770.9717147110569766No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC8760.7903640546758696No Hit
GGAAGTAAAAGTCGTAACAAGGAGACCGAGGAGAAGAAATCCGATAAGAC6730.6072089141516669No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT6550.5909685568638066No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC4670.42134704741282086No Hit
GGAAGTAAAAGTCGTAACAAGGAATTGTTGAAAAAGCTGGTAATTGTCAT3380.30495782018315515No Hit
GGAAGTAAAAGTCGTAACAAGGCCAGCACGCCCATGCCCGCCACCAAAGT2760.24901881174719176No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2240.20210222402670636No Hit
GGAAGTAAAAGTCGTAACAAGGTCGAGCAGAACAAGCTCACCGTCACGGG2230.2011999819551586No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG2210.19939549781206298No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2200.1984932557405152No Hit
GGAAGTAAAAGTCGTAACAAAGAGGATCTTAGCACATGCACATAGAAACA2170.1957865295258718No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGAAGGGGAACCTGCGGAAGGATC2140.1930798033112284No Hit
GGAAGTAAAAGTCGTAACAAGAAAGTGGGTGACTGAGAAATGAGCGGTAT2110.19037307709658502No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2030.18315514052420265No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1990.17954617223801148No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC1880.1696215094509857No Hit
GGAAGTAAAAGTCGTAACAAGGCCGTAGCAGAGCTGGAGCGGCTTCACGA1870.1687192673794379No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1820.16420805702169894No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGAAGCATGTCTACAAGGTTGCATT1780.16059908873550774No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGAAGCATGTATACAAGGTTGCATT1650.1488699418053864No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC1620.146163215590743No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT1560.14074976316145624No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA1550.13984752108990842No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1540.13894527901836062No Hit
GGAAGTAAAAGTCGTAACAAGGTATCTGTAGGTGAACCTGCAGATGGATC1450.13082510037443046No Hit
GGAAGTAAAAGTCGTAACAAGGCGACGCGGAGTTGGCTTTGATGCCCGCG1420.12811837415978708No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1310.11819371137276131No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG1230.11097577480037894No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT1210.10917129065728334No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT1190.10736680651418776No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTGTTCGAGGAGTTGGGGCAGGGCG1190.10736680651418776No Hit
GGAAGTAAAAGTCGTAACAAGGCAGTGCGTCTGCACCCTTTTCCTTTGCA1190.10736680651418776No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG1130.10195335408490099No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA1120.10105111201335318No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1110.10014886994180539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)