FastQCFastQC Report
Sun 21 May 2023
EGAF00007833385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71488
Sequences flagged as poor quality0
Sequence length247-250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4490062.80774395702775No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA875912.25240599820949No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA42115.890499104744852No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG12401.7345568487018799No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC11641.6282452999104746No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA9881.382050134288272No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC8531.1932072515666965No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC8031.123265443151298No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC7120.9959713518352731No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6160.8616830796777081No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG5350.7483773500447628No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT3910.5469449418084154No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3390.47420546105640105No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGGAGGATC2740.38328111011638316No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC2660.37209042076991944No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2620.3664950760966876No Hit
GGAAGTAAAAGTCGTAACAAGGTCTTCGTAGGTGAACCTGCGAAGGGATC1980.2769695613249776No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1900.2657788719785139No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1760.24619516562220234No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC1610.2252126230975828No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1420.19863473589973143No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGTCATATAGAAGTCACTACGATTAA1150.1608661593554163No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC900.1258952551477171No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC840.1175022381378693No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC820.11470456580125335No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAGGTTGCAGGATATGGTGTTCATA820.11470456580125335No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC740.10351387645478963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)