FastQCFastQC Report
Sun 21 May 2023
EGAF00007833409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214884
Sequences flagged as poor quality0
Sequence length247-250
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA9238842.994359747584745No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC8269738.484484652184435No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC208509.70290947674094No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC56742.6404944062843207No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG20730.9647065393421568No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC6250.2908546006217308No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5660.2633979263230394No Hit
GGAAGTAAAAGTCGTAACAAGGTGTGGAAGTGGAACGACCTCGTGCGTGG2550.11866867705366616No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2250.10470765622382308No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAAGGATC2210.10284618677984401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)