FastQCFastQC Report
Wed 8 May 2024
EGAF00007833424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225047
Total Bases53.1 Mbp
Sequences flagged as poor quality0
Sequence length235-238
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT6117627.18365496985074No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4287619.052020244660003No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2598111.544699551649211No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT220139.781512306318236No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG174037.7330513181690925No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG146286.499975560660662No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT55132.4497105049167507No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG35061.5578967948917337No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA22861.0157878132123512No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT19810.8802605677925056No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG13270.5896546054824103No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT12050.5354437073144721No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG11920.5296671361982164No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11550.5132261260981039No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGCTGAAAGTTT11190.4972294676223189No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT10560.46923531528969503No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT7740.34392815722937875No Hit
GCTGCGTTCTTCATCGATGCCCAGTGAAGTGCAGCGGGCAGCATCGGTCA6890.3061582691615529No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGCTGAAAGTTT5940.2639448648504535No Hit
GCTGCGTTCTTCATCGATGCTGAGCAGCGCGAAGTGGCCCGCGAGCTGTA5550.2466151515016863No Hit
GCTGCGTTCTTCATCGATGCTAGTGCCAAGAGATCCATTGTCAAAAGTTG4690.20840091180953313No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG4450.1977364728256764No Hit
GCTGCGTTCTTCATCGATGCGATAAAGGTTGGCATCGTGTTTAATGGGTG4280.19018249521211125No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT3580.15907788150919586No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3420.15196825551995807No Hit
GCTGCGTTCTTCATCGATGCGGTCCGCCATCGCCCCGTCGGAGCCGACGA3320.14752473927668444No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGATATCCGTTGTTGAAAGTTT3290.14619168440370234No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT2870.1275289161819531No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG2860.12708456455762573No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT2860.12708456455762573No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC2810.12486280643598893No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA2720.12086364181704266No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAG2660.11819753207107848No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG2450.10886614796020387No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)