FastQCFastQC Report
Sun 21 May 2023
EGAF00007833425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146398
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC11401777.88152843618083No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC99326.78424568641648No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC77385.285591333214935No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC14040.9590294949384555No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG13810.9433188977991502No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGGTGTGGTGTTCCAAGCAAGGCAGG12980.8866241342094837No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC11540.7882621347286165No Hit
GGAAGTAAAAGTCGTAACAAGGAGATGCAGAAAACGGCAGCGCTTCGGGA9120.6229593300454924No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC7680.5245973305646252No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG6440.43989671990054513No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC6290.4296506782879548No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG4670.3189934288719791No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC3050.20833617945600352No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2990.2042377628109674No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1910.13046626320031696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)