FastQCFastQC Report
Sun 21 May 2023
EGAF00007833433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277173
Sequences flagged as poor quality0
Sequence length247-250
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC14389651.91559062390637No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA4836917.450833955688324No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG222948.043351985943797No Hit
GGAAGTAAAAGTCGTAACAAGGCAGCAGTCTTGCCCTGAACATGGTTTCC116754.212170738131059No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG64372.3223762776316597No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC53651.9356142192782126No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC46091.6628603796185053No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC40041.4445851507903007No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG32971.189509800738167No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC28631.0329288927853724No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGGATCGTCGGCGGGTGCGGCCTTTC16300.5880803685784691No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC8550.30847160437704974No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG8120.2929578277826484No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC7600.2741969816684886No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC7150.2579616340696965No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG5250.18941238865257437No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA4080.14720048489571494No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC3720.13421220681668128No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTCGTGCG3500.12627492576838292No Hit
GGAAGTAAAAGTCGTAACAAGGCGAATATCCGTCACAGTCACTTCCCCAA2800.10101994061470633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)