FastQCFastQC Report
Sun 21 May 2023
EGAF00007833478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310970
Sequences flagged as poor quality0
Sequence length235-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGTCCGCGCT13447243.24275653600026No Hit
GCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC7074322.749139788404026No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA173875.591214586616072No Hit
GCTGCGTTCTTCATCGATGCGCTGGGCTATCCCTTCTGCCGTGGCCGTCT145344.673762742386725No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT77242.483840884972827No Hit
GCTGCGTTCTTCATCGATGCAATGAGCGGAGAAACAACAATCGCCGTTCC73852.374827153744734No Hit
GCTGCGTTCTTCATCGATGCCGCCTCCAGACGGAATCTGGAACTCACAGA61811.9876515419493843No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT53011.704666044956105No Hit
GCTGCGTTCTTCATCGATGCAATGAGCGGAGAAACGACAATCGCCGTTCC47261.5197607486252693No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC44711.4377592693828987No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTGCGCT23550.757307778885423No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA18910.6080972441071486No Hit
GCTGCGTTCTTCATCGATGCACTGATCTCGCAGATGAGCGAGCAGCCCAT18190.584943885262244No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT18140.5833360131202366No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT15720.5055150014470849No Hit
GCTGCGTTCTTCATCGATGCGTCGGACAGTCACAATCAACTCACATGCGG14360.4617808791844873No Hit
TCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGTCCGCGCT10420.3350805543943146No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT9390.3019583882689649No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA7240.23281988616265237No Hit
GCTGCGTTCTTCATCGATGCCGCTGCGGAGGAAGCACTGCCGCTTGAGAA7230.2324983117342509No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT6620.2128822716017622No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6200.19937614560890118No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTCCTTGTCT5930.19069363604206194No Hit
TCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC5780.18587001961604013No Hit
GCTGCGTTCTTCATCGATGCCCAGTGCAGCAGCTGAACGGAATATCGCCC5470.17590121233559508No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT5190.16689712834035436No Hit
GCTGCGTTCTTCATCGATTCGAAACGCCTAGGCATCCGCCGTATGCCCTT4990.1604656397723253No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG4690.1508184069202817No Hit
GATGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGTCCGCGCT3300.10611956137247967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)