FastQCFastQC Report
Sun 21 May 2023
EGAF00007833483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275811
Sequences flagged as poor quality0
Sequence length247-250
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC17901264.90386532806886No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA4991618.0979003738067No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG132974.821054997806469No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC55742.0209491282073593No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC50951.8472794776132933No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC47111.7080537034418495No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG13590.4927287164036242No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC7520.2726504744190768No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG5750.20847609413692708No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC4840.1754824861952569No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC4380.15880439866430274No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCTAATCGACGCAATCG3650.13233699888691894No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC3380.12254768664048932No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2920.10586959910953515No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2880.10441933062858262No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2820.10224392790715382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)