FastQCFastQC Report
Sun 21 May 2023
EGAF00007833488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49222
Sequences flagged as poor quality0
Sequence length236-238
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1898438.56811994636545No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT803116.31587501523709No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT38097.738409654219658No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG17373.5289098370647274No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC11052.244931128357239No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT10022.035675104628012No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG8661.7593758888301978No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT6591.338832229490878No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG6211.2616309780179593No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5901.1986510097111047No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT5441.1051968631912559No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT5041.023932387956605No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT4200.8532769899638373No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC3940.800455081061314No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG3070.6237048474259478No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA2440.49571329893137217No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA2230.4530494494331803No Hit
GCTGCGTTCTTCATCGATGCCTAGTGCCAAGGCATCCACCGTATGCCCTT2040.414448823696721No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2020.4103855999349884No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1990.4042907642923896No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1830.3717849741985291No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1810.36772175043679656No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT1810.36772175043679656No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTCCACTTCGCAAATCAAAATAA1710.34740563162813376No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1590.32302628905773845No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT1590.32302628905773845No Hit
GCTGCGTTCTTCATCGATGCGACGTAACACAAGGTCGATACCTTTTTCTC1400.2844256633212791No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1320.2681727682743489No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT1210.2458250375848198No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1170.23769859006135466No Hit
GCTGCGTTCTTCATCGATGCCTTTCGCCGACAAACAACTAACGTAGATAT940.19097151680143026No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT920.1869082930396977No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTTTTGAAAGTTT870.1767502336353663No Hit
GCTGCGTTCTTCATCGATCGGATAAGTCTCCGACACCGGTTCCGTTGCAA770.1564341148267035No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGAGATCCGTTGTTGAAAGTTT770.1564341148267035No Hit
GCTGCGTTCTTCATCGATGCGTGCGGGGAAGTTGTAGAGAATAATCGGCA720.14627605542237213No Hit
GCTGCGTTCTTCATCGATGCCCGGCTTCCTTTTTCTCTTTATCGAAAAAA710.14424444354150584No Hit
GCTGCGTTCTTCATCGATGCCATTGAAAGTATACCAATAGCTTTCTCTAT700.14221283166063955No Hit
GCTGCGTTCTTCATCGATGCGTTTTTATTCTCTGTTTTTTTCTTTACGCT660.13408638413717444No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT650.13205477225630816No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA640.1300231603754419No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG620.12595993661370933No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACTTGG620.12595993661370933No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA610.12392832473284304No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG610.12392832473284304No Hit
GCTGCGTTCTTCATCGATGCGGTACCGACTTCGAAGACTTGCAGGATGGC600.12189671285197676No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAATTT570.11580187720937792No Hit
GCTGCGTTCTTCATCGATGCCAGCCACAAAACCTGCGGCAACAGTTGTGT540.10970704156677909No Hit
GCTGCGTTCTTCATCGATGCCAGGATTTTGGTGGAGGATGACGGGATCGA530.1076754296859128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)