FastQCFastQC Report
Sun 21 May 2023
EGAF00007833497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310510
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC22404272.15290972915525No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG287709.265402080448295No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC52661.695919616115423No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA49731.6015587259669575No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG44031.417989758783936No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGAC39251.2640494670058935No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGCGAAGACGTCGAGAAGGAAGCGGC27280.8785546359215484No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGCTGGATC25870.833145470355222No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC20970.6753405687417474No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC20870.6721200605455541No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCAGAAAATG10680.3439502753534508No Hit
GGAAGTAAAAGTCGTAACAAGGTGTTGAAACACTTTGACGTCAAAAACCT8500.27374319667643554No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGACCCTGCGGCTGGATC8500.27374319667643554No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC8320.26794628192328745No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGGAAGAAGCGGC8090.2605391130720428No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC8040.2589288589739461No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC7830.25216579176194004No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCAAGAAGGAAGCGGC7700.24797913110688866No Hit
GGACGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC6980.22479147209429648No Hit
GGAAGTAAAAGTCGTAACAAGGCCGTGAAGTAAGCGGGAAGCATATTGAT6730.2167402016038131No Hit
GGAAGTAAAAGTCGTAACAAGGGCAATAAGAAGAAGGAGAAGGGCGTCGA6660.2144858458664777No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA4900.1578049016134746No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAAAAGCAACTGAAAGGCAACAAGGG4630.14910952948375253No Hit
GGAAGTAAAAGTCGTAACAAGGAGGTGGGAGAAAGCTACATTGTCTTCGT4190.13493929342050176No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC3970.12785417538887636No Hit
GGAAGTAAAAGTCGTAACAAGTCCGTAGTGGTGGCGCATAACGTGGAAGC3840.12366751473382499No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGGC3320.10692087211361953No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGAGACCTCGAAGACCTGATGGAAAA3140.10112395736047149No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC3110.10015780490161348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)