FastQCFastQC Report
Sun 21 May 2023
EGAF00007833516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82287
Sequences flagged as poor quality0
Sequence length235-238
%GC62

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4474654.37796978866649No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT1176814.301165433178994No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT43185.247487452453No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG15021.8253187016175092No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT13311.6175094486370871No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT11091.3477219973993462No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT8981.0913023928445562No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA8861.076719287372246No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATTAAAATAA8010.9734222902767145No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT7920.9624849611724816No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT6910.839743823447203No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5890.7157874269325653No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA5360.651378711096528No Hit
GCTGCGTTCTTCATCGATGCTTCTTGAATAGTAACCTTTTCTAAGCACTA5180.6295040528880625No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG4110.49947136242662876No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3740.45450678722033855No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3420.4156185059608444No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3060.3718691895439134No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG2250.2734332276058187No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT2250.2734332276058187No Hit
GCTGCGTTCTTCATCGATGCTCATTATCTTTTTTTTTTGGTAATAGTTAT1890.22968391118888767No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1830.2223923584527325No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT1810.2199618408740141No Hit
GCTGCGTTCTTCATCGATGCATTCAATATTGCTATTTACTTTGCAAATTA1470.17864304203580153No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1380.16770571293156877No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1340.162844677774132No Hit
GCTGCGTTCTTCATCGATGCGAAGAAGGGCATGCTGCACCGCGCCGACTT1250.15190734866989927No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1230.14947683109118087No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1190.14461579593374407No Hit
GCTGCGTTCTTCATCGATGCTTCATCATAGTCGCCATAACCATGAAGGCT1170.1421852783550257No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG980.119095361357201No Hit
GCTGCGTTCTTCATCGATGCTGGGGCTGGCATTGATAGATTTGGCTACAG960.11666484377848263No Hit
GCTGCGTTCTTCATCGATGCCGCCAAAATCGGCAGGGAACTGGGCTGGAA950.11544958498912344No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG940.11423432619976424No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG930.11301906741040504No Hit
GCTGCGTTCTTCATCGATGCGTGGTTTTCGCAAATTAAAGCAGTCTGATT930.11301906741040504No Hit
GCTGCGTTCTTCATCGATGCCCAGCGTGAGCTGCATGATGACGAGCTTCT920.11180380862104584No Hit
GCTGCGTTCTTCATCGATGCGGTCGCGGTCGGCGCAGTCCACTACGAAGA890.10815803225296826No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT830.1008664795168131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)