FastQCFastQC Report
Sun 21 May 2023
EGAF00007833521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97261
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT2151422.119863048909636No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG2061521.195546005079116No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1853619.057998581137355No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC88659.114650270920514No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA50125.153144631455568No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC24152.483009633871747No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG22212.2835463340907456No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG17491.7982541820462465No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC16021.6471144652018794No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC12331.2677229310823455No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG9600.9870348855142349No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGGC7070.7269100667276709No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC5510.5665168978316077No Hit
GGAAGTAAAAGTCGTAACAAGGATAGGTGAAATCTCAGAGAAAGCGTAGA3430.3526593393035235No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2860.2940541429761158No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2750.2827443682462652No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTGATTT1680.17273110496499108No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1430.1470270714880579No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCATGG1350.13880178077543928No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTGTGGTGTTCCAAGCAAGGCAGG1180.12132303801112469No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTCGTGCG1180.12132303801112469No Hit
GGAAGTAAAAGTCGTAACAAGGGCTCAAGGAAACTGAACAAGCTACGGGA1120.11515406997666075No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTGTTCGAGGAGTTGAGGCAGGGCG1080.11104142462035142No Hit
GGAAGTAAAAGTCGTAACAAGGATCCGGAGAAGAAGATTGCAGATGTGCT1050.10795694060311944No Hit
GGAAGTAAAAGTCGTAACAAGGGAGATGGGGATGCAGGGTGCTCCTGTGG1050.10795694060311944No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGAGTT1020.10487245658588745No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG1000.1028161339077328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)