FastQCFastQC Report
Sun 21 May 2023
EGAF00007833527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347379
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC12560836.15877758874313No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG9967628.693732206034333No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC5731716.4998459895388No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTGATTT69592.0032874756389996No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTCGTGCG27310.786173027154779No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA25340.7294626330319335No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT18420.5302565785496532No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC17640.5078027169172575No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA16880.4859245953267181No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC14170.40791181965518936No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC13250.3814277777297994No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTTGTGCG12990.37394315718566756No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTAACTT10350.2979454716606358No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC10320.2970818615978513No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC9910.2852791907397972No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA9550.27491586998638373No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG8570.24670460793542498No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA7710.22194778613560404No Hit
GGAAGTAAAAGTCGTAACAAGGGGAATGAAGAGGAGCATAAGTAAATAAT7320.21072085531940618No Hit
GGAAGTAAAAGTCGTAACAAGGATAGGTGAAATCTCAGAGAAAGGGTAGA7210.20755428508919652No Hit
GGAAGTAAAAGTCGTAACAAGGGAGATGGGGATGCAGGGTGCTCCTGTGG6990.20122114462877722No Hit
GGAAGTAAAAGTCGTAACAAGGATAGGTGAAATCTCAGAGAAAGCGTAGA6290.18107024316380668No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGAGGGCAAGGAAAAGTGGAGGAG6250.17991876308009408No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC5530.15919212157326723No Hit
GGAAGTAAAAGTCGTAACAAGGCAGACAAGCGTATTCTCGAATTGCTAAC5000.14393501046407528No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC4990.1436471404431471No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG4880.14048057021293744No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4680.13472316979437443No Hit
GGAAGTAAAAGTCGTAACAAGCCGGGAGAAAGAGGAAGCGCCGGCGCCAA4540.13069298950138034No Hit
GGAAGTAAAAGTCGTAACAAGGACGAAGTGCACCTGGCTGACGCCATCAT4410.12695067922931438No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC4100.1180267085805417No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG3720.107087647785272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)