FastQCFastQC Report
Sun 21 May 2023
EGAF00007833542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125387
Sequences flagged as poor quality0
Sequence length235-238
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT7384058.889677558279566No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1294310.322441720433538No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT65385.214256661376378No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT43953.505148061601283No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA37312.975587580849689No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA34632.761849314522239No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT14441.1516345394658138No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG12921.0304098511009911No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT8410.6707234402290508No Hit
GCTGCGTTCTTCATCGATGCCGTTCAGGTAGCAGGAAAGCTTCCTATTCA8230.6563678850279534No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG7810.6228715895587261No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA5450.43465431025544915No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4690.3740419660730379No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3890.31023949851260496No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT3850.3070493751345833No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG3830.3054543134455725No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3570.2847185114884318No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT3540.28232591895491554No Hit
GCTGCGTTCTTCATCGATGCTAGAGCCAAGAGATCCGTTGTTGAAAGTTT2840.2264987598395368No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT2680.21373826632745024No Hit
GCTGCGTTCTTCATCGATGCTGTGGTGGTGACGGTGGCTGCTGCGCTGGG2270.18103950170272834No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2250.1794444400137175No Hit
TCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2190.17465925494668508No Hit
GCTGCGTTCTTCATCGATGCGGACGTACTTGCCGATTCGCTCGCACTTGT1980.15791110721207144No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGTCAAACT1910.15232839130053355No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGACGCTGCGTTCTTCATCGA1900.15153086045602812No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT1820.14515061369998486No Hit
GCTGCGTTCTTCATCGATGTCGCTTCCGAGGCCGGACGATCGGGCAAACT1660.13239012018789825No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGACAAACT1640.13079505849888745No Hit
GCTGCGTTCTTCATCGATGCTGCACAGAGCCTGCATCCTCCTCCTCCTCG1630.12999752765438202No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGAGGCTGCGTTCTTCATCGA1450.11564197245328464No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCAACGCCGGACGATCGGGCAAACT1380.11005925654174675No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCTAGGCCGGACGATCGGGCAAACT1350.10766666400823051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)