FastQCFastQC Report
Sun 21 May 2023
EGAF00007833544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64927
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2500638.51402344171146No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT55668.572704729927457No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA54368.37247986199886No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT38665.954379533938115No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACACGTGT25653.9505906633603893No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA22823.5147165277927517No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT20523.1604725306883115No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG13972.1516472345865356No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT7661.1797865294869623No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT6561.0103654873935342No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC4990.7685554545874598No Hit
GCTGCGTTCTTCATCGATGCTGTCGTGGTGACGGTGGCCGCGGCGCTGGG4980.7670152632957014No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4960.7639348807121845No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3730.5744913518258967No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3290.5067229349885256No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3190.4913210220709412No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCGGCTGCGTTCTTCATCGA3070.47283872656983994No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT3020.4651377701110478No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCCGCTGCGTTCTTCATCGA2910.44819566590170495No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT2830.4358741355676375No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG2740.42201241394181155No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT2660.4096908836077441No Hit
GCTGCGTTCTTCATCGATGCCATCCCGGACGACTCCATCATCGCTCAATA2590.398909544565435No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCTGCTGCGTTCTTCATCGA2400.36964591002202474No Hit
GCTGCGTTCTTCATCGATGGCACCAGTGTCACCTTTAGCACCGGTATCAC2370.3650253361467494No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1740.26799328476596795No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG1550.23872965022255763No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1530.23564926763904076No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC1510.2325688850555239No Hit
GCTGCGTTCTTCATCGATGCTCACTGACGATCCCTACCACCTCGCTCCCT1500.23102869376376545No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCGGCTGCGTTCTTCATCGG1450.22332773730497327No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT1440.22178754601321482No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1430.2202473547214564No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCAGCTGCGTTCTTCATCGA1270.19560429405332141No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1250.19252391146980455No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1210.18636314630277082No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC1120.17250142467694488No Hit
GCTGCGTTCTTCATCGATGCCTGCGGATGCAATTGCCTCGACACTCGGGT1040.16017989434287738No Hit
GCTGCGTTCTTCATCGATGCGCTCTTCAGCCTTCTGGATCTTAGCCTTCT1030.15863970305111896No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCTGCTGCGTTCTTCATCGG990.1524789378840852No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCCGCTGCGTTCTTCATCGG960.1478583640088099No Hit
GCTGCGTTCTTCATCGATGCGATAAAGGTTGGCATCGTGTTTAATGGGTG940.14477798142529302No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT920.14169759884177616No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG910.1401574075500177No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTGTCAAGAACGAGTTCGTAAT880.1355368336747424No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG860.13245645109122553No Hit
GCTGCGTTCTTCATCGATGCTTCATCATAGTCGCCATAACCATGAAGGCT840.12937606850770866No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT800.1232153033406749No Hit
GCTGCGTTCTTCATCGATGCCTTTCGCCGACAAACAGCTAACGTAGATAT780.12013492075715804No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG760.11705453817364116No Hit
GCTGCGTTCTTCATCGATGCGCTGGGCTATCCCTTCTGCCGTGGCCGTCT750.11551434688188272No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG750.11551434688188272No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG720.11089377300660741No Hit
GCTGCGTTCTTCATCGATGCGGTTATGACGTCAGCTGCGTTCTTCATCGG720.11089377300660741No Hit
GCTGCGTTCTTCATCGATGCGGATGTCGACCGCCTCAAGATCGACCGCGG710.10935358171484898No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG700.10781339042309054No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG700.10781339042309054No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG680.10473300783957366No Hit
GCTGCGTTCTTCATCGAAACGTATATAGCTTCCGTCTTTTCTTCTTGTTT680.10473300783957366No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG670.10319281654781523No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATACGTTGCCGAGAGTCG650.10011243396429836No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT650.10011243396429836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)