FastQCFastQC Report
Sun 21 May 2023
EGAF00007833547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29588
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC593720.06556712180614No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGATGTCGAGGAAGAAGCGGC386713.069487630120319No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC315410.659726916317426No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA26969.111802081925106No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGGC12074.0793564958767075No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA11603.9205083141814248No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC9723.2851155874002975No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG8832.984317966743274No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC8342.818710287954576No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7292.463836690550223No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT6452.1799378126267404No Hit
GGAAGTAAAAGTCGTAACAAGTGGCAAGAAGAATACGATTAGCACTTGAC5671.9163174259835067No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC4491.517507097471948No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC3001.0139245640124375No Hit
GGAAGTAAAAGTCGTAACAAGGCACTGAAGGAGAAGGGAGGGAAGACGAT2440.8246586453967826No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG1490.5035825334595105No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1470.4968230363660944No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC1430.4833040421792618No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGGATCGTCGGCGGGTGCGGCCTTTC1400.47316479653913746No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG1370.4630255508990131No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1260.42584831688522373No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGATGTAGAGGAAGAAGCGGC1210.4089495741516831No Hit
GGAAGTAAAAGTCGTAACAAGGGGTGTCGGTGTAGGTCATTGGTTTTCCT1200.405569825604975No Hit
GGAAGTAAAAGTCGTAACAACTGGAGAAGTAAGACTATCATACGCACATC1030.34811410031093687No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGAGACCTCGAAGACCTGATGGAAAA980.33121535757739623No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC950.32107611193727187No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG920.3109368662971475No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC780.26362038664323373No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA780.26362038664323373No Hit
GGAAGTAAAAGTCGTAACAAGCAAGAAGCGCAGCAGTTGAAAGAGAAAAA720.243341895362985No Hit
GGAAGTAAAAGTCGTAACAAGGCTGCCAGCTTCGGCTTGACCGAACGAAT700.23658239826956873No Hit
GGAAGTAAAAGTCGTAACAAGCCAATGGTTAGCACTAAACGCTTCAAAGC690.2332026497228606No Hit
GGAAGTAAAAGTCGTAACAAGGGAACCGCTTTTACGCAGGAGGAGCGCCA650.21968365553602814No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT640.21630390698932No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA630.21292415844261187No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG600.2027849128024875No Hit
GGAAGTAAAAGTCGTAACAAGGTCAATAGCAAGGGCAAGCGCCGCATCAA550.18588617006894687No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGTCTGGATC510.17236717588211437No Hit
GGAAGTAAAAGTCGTAACAAGGGCAAGGGAGCAGGAAATAAAGAAAAAGA470.15884818169528186No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT470.15884818169528186No Hit
GGAAGTAAAAGTCGTAACAAGGGCATAAGGGGGAGGCTGGCGAAGGCGAA460.15546843314857375No Hit
GGAAGTAAAAGTCGTAACAAGGACAACTGGAAGCAGTACATCGACGGACT460.15546843314857375No Hit
GGAAGTAAAAGTCGTAACAAGGTAGGAGAAGAAGAGCCCTGGCGAGGCAA450.15208868460186561No Hit
GGAAGTAAAAGTCGTAACAAGGAGCGGGGAATAGCAAGATGCGTCACTTC440.1487089360551575No Hit
GGAAGTAAAAGTCGTAACAAGGGAAAGAAGGTTATCATCTTCAAGTACAA420.14194943896174125No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAAAAGGTTTTGGTGGGCAA410.13856969041503311No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG400.135189941868325No Hit
GGAAGTAAAAGTCGTAACAAGGGCATGACCTATACGTACAACCCCTACGA400.135189941868325No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCAAGGTTGTCCCACAATCGGATTTC390.13181019332161686No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG380.12843044477490873No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA370.12505069622820061No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAGGTTTTGGTGGGCAA370.12505069622820061No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTGGGCAACCGATTGTACCCTTACAA360.1216709476814925No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC360.1216709476814925No Hit
GGAAGTAAAAGTCGTAACAGGTCCAACGATTCAAACACCAACTGATAGAC320.10815195349466No Hit
GGAAGTAAAAGTCGTAACAAGGACTGTCAGAGCACCAGCACAGACCGAGA320.10815195349466No Hit
GGAAGTAAAAGTCGTAACAAGGTGGTGGAGGCCATCAACAACGGCTTCCT320.10815195349466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)