FastQCFastQC Report
Sun 21 May 2023
EGAF00007833548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29588
Sequences flagged as poor quality0
Sequence length235-238
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGCTGGGCTATCCCTTCTGCCGTGGCCGTCT490416.574286873056646No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT359812.160335271055834No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT26198.85156144382858No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG18876.377585507638233No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA11984.048938758956334No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC11723.961065296741922No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT10323.4879005002027847No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA8372.828849533594701No Hit
GCTGCGTTCTTCATCGATGCATTCAATATTGCTATTTACTTTGCAAATTA8372.828849533594701No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC8232.781533053940787No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT6462.1833175611734488No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG5431.8352034608625118No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTGGTCATTTACTACAAACAAATTTC5361.811545221035555No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG4381.4803298634581585No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT4071.3755576585102067No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG3081.0409625523861024No Hit
GCTGCGTTCTTCATCGATGCGCCTTGAGGTTGTCCATCGTCATCATGGCA2510.8483168852237393No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA2510.8483168852237393No Hit
GCTGCGTTCTTCATCGATGCACTGGGCTATCCCTTCTGCCGTGGCCGTCT1880.6353927267811275No Hit
GCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC1460.49344328781938623No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA1460.49344328781938623No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT1430.4833040421792618No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT1360.459645802352305No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1300.4393673110720563No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT1180.39881032851155873No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1170.39543057996485065No Hit
GCTGCGTTCTTCATCGATGCCGACGAATATGCGCAAAACCCCGGCCTGCA1110.37515208868460187No Hit
GCTGCGTTCTTCATCGATGCGCTGGGCTATCCCTTCTGCCGTTGCCGTCT1060.35825334595106123No Hit
GCTGCGTTCTTCATCGATGCGACCCTTGGTAGCAGTGTCTGTCTTTGGAA1040.35149384885764495No Hit
GCTGCGTTCTTCATCGTTTCAAAACGCCTAGGCATCCGCCGTATGCCCTT1030.34811410031093687No Hit
GCTGCGTTCTTCATCGATGCCCTCTATCAGACGAATGGTAAAGGGGATAT970.3278356090306881No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT920.3109368662971475No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT880.297417872110315No Hit
GCTGCGTTCTTCATCGATGCAATGAGCGGAGAAACGACAATCGCCGTTCC880.297417872110315No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCGTCTTTTCTTCTTGTTT800.27037988373665No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT770.2602406380965256No Hit
GCTGCGTTCTTCATCGATGCGACTATGGTAAGGCGCAGAAACAGTTTGGC750.25348114100310937No Hit
GCTGCGTTCTTCATCGATGCTGATTTCGCCGTTGTGAATACTGCCGCTAA700.23658239826956873No Hit
GCTGCGTTCTTCATCGATGCGCAGGTCATAGCCCATGGCAGCCACAGCTG700.23658239826956873No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT690.2332026497228606No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGCAATGGCAACTGGACCAAAATCC690.2332026497228606No Hit
GCTGCGTTCTTCATCGAGGTCTCAAGGTCCTCAGGACGATCGGCGGAGAG670.22644315262944437No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT630.21292415844261187No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG630.21292415844261187No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT570.19264566716236312No Hit
GCTGCGTTCTTCATCGATGCAGTCCTGGTCATTTACTACAAACAAATTTC560.189265918615655No Hit
GCTGCGTTCTTCATCGAAACGAATATAGGAACCGTCTTGACGGCGGATAC560.189265918615655No Hit
GCTGCGTTCTTCATCGATGCGCTCGCATAGCAAATTCATTTCAGCCCCAT540.18250642152223873No Hit
GCTGCGTTCTTCATCGATTACCGATAAGTGTGAGTACCTAGACCCCTTTT520.17574692442882248No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTCT490.16560767878869811No Hit
GCTGCGTTCTTCATCGATGCCGCCGCCGACGAAGGTGACGGCCAACAGGA470.15884818169528186No Hit
GCTGCGTTCTTCATCGATGCACGCATCAACCAACGACTTATAGAAAACAT470.15884818169528186No Hit
GCTGCGTTCTTCATCGATGCCATCCGCGACGAATAAGTCCACTGCTACCA470.15884818169528186No Hit
GCTGCGTTCTTCATCGATGCGGTCCACGTTCCTGTTGTGGTCCTCCGCCA470.15884818169528186No Hit
GCTGCGTTCTTCATCGATGCCTTCTAGCTCTTCTGTAGTGGGGGCTTGGG440.1487089360551575No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTCGCAAATCA440.1487089360551575No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT430.1453291875084494No Hit
GCTGCGTTCTTCATCGATGCCCAGCGTGAGCTGCATGATGATGAGCTTCT420.14194943896174125No Hit
GCTGCGTTCTTCATCGATGCTGTCGTCGTGACGGTGGCCGCGGCGCTGGG410.13856969041503311No Hit
GCTGCGTTCTTCATCGATGCTGTCGACCAGCAGGAACTCGCACTCGGGGA400.135189941868325No Hit
GCTGCGTTCTTCATCGATGCACGGGAGCCCGTCCTTAACAAATACAAAGA380.12843044477490873No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG370.12505069622820061No Hit
GCTGCGTTCTTCATCGAGGTCGATGAAGGAGTCATAGCGCTCGGAGCGCA370.12505069622820061No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG360.1216709476814925No Hit
GCTGCGTTCTTCATCGATGCCTCATCATAGTCGCCATAACCATGAAGGCT340.11491145058807625No Hit
GCTGCGTTCTTCATCGATTTAACTATAATCGTAAATTGTCTTTGACTAGG340.11491145058807625No Hit
GCTGCGTTCTTCATCGATGCTGCCATTGCCCTCGATACGATGAATCATCG310.10477220494795186No Hit
GCTGCGTTCTTCATCGATGCCACTTAAACGAACCATCAGCGAATAGCCGT300.10139245640124375No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG300.10139245640124375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)