FastQCFastQC Report
Sun 21 May 2023
EGAF00007833551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148867
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC8340256.024505095152044No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2114714.205297345953099No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1699211.414215373454157No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC39792.672855636239059No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC21121.4187160351185957No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC16941.1379284865013737No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA12480.8383322025700792No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT10130.6804731740412583No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC9920.666366622555704No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC6370.42789872839514465No Hit
GGAAGTAAAAGTCGTAACAAGGCGATCTCAAAACAGTGACGTTTTGCGTA6280.42185306347276424No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT4760.31974850033922897No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA3880.2606353322092875No Hit
GGAAGTAAAAGTCGTAACAAGGGAAAGAAGGTTATCATCTTCAAGTACAA3600.24182659689521518No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTGGGGCAGAAGGTGGAAAATGCGCG3600.24182659689521518No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3470.2330939697851102No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3140.21092653173638212No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACAAGCACGATGCCGAGGATTTT3010.20219390462627712No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAGTGAACCGCGGGAGCCTCAGAAGG2850.1914460558753787No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG2830.1901025747815164No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT2590.17398080165516872No Hit
GGAAGTAAAAGTCGTAACAAGGTGGGTGATGTGGTCTACTCCCAGGCCGG2450.16457643399813254No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC2420.16256121235733909No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2320.15584380688802757No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT2130.14308073649633565No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1850.12427200118226336No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTTTAGGTGAACCTGCAGAAGGATC1590.10680674696205336No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1580.10613500641512223No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA1530.10277630368046645No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)