FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007833555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138302
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC4681833.85200503246518No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3454324.976500701363683No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC72325.229136238087663No Hit
GGAAGTAAAAGTCGTAACAAGGGGCGGCGTGTCTCTGCCGACAGGTATTC44363.207473500021692No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA44093.187951005769982No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC36222.6189064510997673No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC27571.9934635797023905No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC25991.879220835562754No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC25771.8633136180243235No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG23531.7013492212693961No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA17331.2530549088227212No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA14521.0498763575364058No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC9970.7208861766279592No Hit
GGAAGTAAAAGTCGTAACAAGGTTCAGCTGGTCGGCGACGATCTCTTTGT8520.6160431519428496No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC8200.59290538097786No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCAGCAGGTTTCCA5550.4012957151740394No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC5500.3976804384607598No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTTATGGCTCAAGCAGAGCAAAA4200.3036832439154893No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA4050.29283741377565037No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG3990.28849908171971483No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGTGCTTGGTTGGGGGTGGTTGCCAT3420.24728492718832698No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA3140.22703937759396106No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAGTGAACCGCGGGAGCCTCAGAAGG3100.22414715622333733No Hit
GGAAGTAAAAGTCGTAACAAGGGAATCGGGGGGATTTCGCCGGGGCGGTA3000.21691660279677805No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAAAACGGTGAATGTGAGCGAAGAAG2870.20751688334225105No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2750.1988402192303799No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGCTGGATC2660.19233272114647654No Hit
GGAAGTAAAAGTCGTAACAAGGGGAATCGGGGGGATTTCGCCGGGGCGGT2610.1887174444331969No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGAAGCGCACGGGCATGAAGTAGGCT2610.1887174444331969No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCTGCTGGATC2580.18654827840522914No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2550.18437911237726134No Hit
GGAAGTAAAAGTCGTAACAAGGGGCCGAGGGTGGTGATCAGGGCGAGGGA2410.17425633758007839No Hit
GGAAGTAAAAGTCGTAACAAGGCTTGGAGTTGTTGGGCATCGATGAAGAA2320.16774883949617503No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA2290.16557967346820726No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGTTGCGGCTGGATC2070.14967245592977688No Hit
GGAAGTAAAAGTCGTAACAGGCGTTAAGTTTACAGCATGAAGAGAACAAA2040.14750328990180908No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT2010.14533412387384131No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACATGGGAACAGGTCCG2010.14533412387384131No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCTATCGCAGCGGGCTTCCA1940.1402727364752498No Hit
GGAAGTAAAAGTCGTAACAAGGTACGGACGCATCGATGAAGAACGCAGCC1910.13810357044728205No Hit
GGAAGTAAAAGTCGTAACAAGGAGGCTAAGCGCATCGTCGTACAGTCGAT1890.13665745976197016No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGTCTGGATC1860.13448829373400242No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTTACCGGAAGGTGCGGCTGGATC1830.13231912770603463No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTGTGGTGTTCCAAGCAAGGCAGG1770.12798079565009907No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1610.11641191016760423No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA1610.11641191016760423No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT1580.11424274413963645No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCAAGGTTGTCCCACAATCGGATTTC1410.1019508033144857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)