FastQCFastQC Report
Sun 21 May 2023
EGAF00007833556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138302
Sequences flagged as poor quality0
Sequence length236-238
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT4280730.95183005307226No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1625911.756156816242715No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT80745.837948836603954No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACGGCTGCGTTCTTCATCGA42853.0982921432806467No Hit
GCTGCGTTCTTCATCGATGCACACCCCCAAATCACCCACGCCCGCGCGAT40332.9160821969313533No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT39872.88282165116918No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT34862.520570924498561No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT30992.2407485068907174No Hit
GCTGCGTTCTTCATCGATGCGATAAAGGTTGGCATCGTGTTTAATGGGTG25161.819207242122312No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA24161.7469017078567195No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT23331.6868881144162775No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT17751.28342323321427No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACTGCTGCGTTCTTCATCGA17471.2631776836199042No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACCGCTGCGTTCTTCATCGA16991.2284710271724197No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT15591.12724327920059No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACGGCTGCGTTCTTCATCGG12580.909603621061156No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACCATGTGTT12300.8893580714667901No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACAGCTGCGTTCTTCATCGA11500.831513644054316No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT10430.7541467223901317No Hit
GCTGCGTTCTTCATCGATGCGTCCGTACCTTGGCTGCGTTCTTCATCGAT10330.7469161689635725No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT10240.7404086708796691No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9360.6767798007259476No Hit
GCTGCGTTCTTCATCGATGCGAATCAGCTGGTTGTACTTCGCAACGCGCT9330.6746106346979798No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT9270.6702723026420442No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACACGTGT8540.6174892626281615No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA6420.46420152998510505No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGGGAGTCG6260.45263264450261026No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG6110.44178681436277134No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT6000.4338332055935561No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTG5640.4078032132579428No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACTGCTGCGTTCTTCATCGG5420.3918959957195124No Hit
GCTGCGTTCTTCATCGATGCGACCGCTCTCGCCCTCGGCGCCTGCGGCTC5340.38611155297826494No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG5240.3788809995517057No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAATACTGTAATGTCTTC5050.3651429480412431No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT4860.35140489653078044No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACAGCTGCGTTCTTCATCGG4500.3253749041951671No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACCGCTGCGTTCTTCATCGG4460.32248268282454334No Hit
GCTGCGTTCTTCATCGATGCCGGCAACGTCTGGACCACACGCGGCAGTGT3970.28705297103440297No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT3850.27837630692253185No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG3740.27042269815331665No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG3680.26608436609738106No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC3540.25596159130019813No Hit
GCTGCGTTCTTCATCGATGCGTTTTGGTAAGGGTAACTGGACCAAAATAC3520.25451548061488627No Hit
GCTGCGTTCTTCATCGATGCCATGCACTACATGGCGTCCACCGGCCTGCT3400.24583881650301515No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACTAATGTGT3110.22487021156599327No Hit
GCTGCGTTCTTCATCGATGCCTGCGGATGCAATTGCCTCGACACTCGGGT2920.21113216005553068No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2770.20028632991569176No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA2600.18799438909054098No Hit
GCTGCGTTCTTCATCGATGCTGATTCAGTTGAGGTGACGAGCGCGCCGGA2440.17642550360804618No Hit
GCTGCGTTCTTCATCGATGCCGAGGCGTTCGCTGGCCGCTATGAGCGTAT2300.1663027288108632No Hit
GCTGCGTTCTTCATCGATGCGTCCGTACCTTGGCTGCGTTCTTCATCGGC2220.16051828606961577No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAACACTGTAATGTCTTC2200.15907217538430393No Hit
GCTGCGTTCTTCATCGATGCGTTCAGGTGTACGGCTGCGTTCTTCATCCT2150.1554568986710243No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT2030.14678023455915315No Hit
GCTGCGTTCTTCATCGATGGTCCGTACCTTGGCTGCGTTCTTCATCGATG1930.1395496811325939No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1880.13593440441931426No Hit
GCTGCGTTCTTCATCGATGCGGGCGGGGTGGATACGACCGTCTTGGACGA1840.13304218304869053No Hit
GCTGCGTTCTTCATCGAAACGTATATAGCTTCCGTCTTTTCTTCTTGTTT1750.1265346849647872No Hit
GCTGCGTTCTTCATCGATGCCATCCGCGACGAATAAGTCCACTGCTGCCG1610.11641191016760423No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAAAAACACACATGTGT1460.10556608002776534No Hit
GCTGCGTTCTTCATCGATGCTGTCCATGGTCACGCGATGCTCTCCTCGAT1450.10484302468510939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)