FastQCFastQC Report
Sun 21 May 2023
EGAF00007833574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278038
Sequences flagged as poor quality0
Sequence length235-238
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG11415141.055898833972336No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT277179.968781245729001No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCAACGCCGGACGATCGGGCAAACT189786.825685697638452No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT158125.686992425495795No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT111234.000532301340105No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT103553.7243110653939393No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT103233.7128018472295152No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG70472.534545637646653No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGAT68432.4611743718484522No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGATG61562.214085844380984No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG55752.0051216020831686No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG34261.2322056697285981No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTG24250.8721829390227235No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT22130.7959343686834174No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT17200.6186204763377668No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT11180.40210330961954843No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT9570.3441975557297923No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGGC8380.3013976506808422No Hit
GCTGCGTTCTTCATCGATGCAGGTATCGAGCATGCCCTCAAGGCCTTCGA8190.2945640523957157No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGGCT7860.2826951711636539No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG6650.23917593997942727No Hit
GCTGCGTTCTTCATCGATGCGATAAAGGTTGGCATCATGTTTAATGGGTG6650.23917593997942727No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT6470.23270200476193903No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT6240.2244297542062596No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT6020.21651716671821836No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA5660.20356929628324189No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT5460.19637603493047714No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGCC3990.14350556398765635No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG3990.14350556398765635No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACACGTGT3680.13235600889087104No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG3420.12300476913227687No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG3310.11904847538825629No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGCCT3310.11904847538825629No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3300.11868881232061804No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG3260.1172501600500651No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTAAAAGTTT3070.11041656176493861No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2990.10753925722383272No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT2970.10681993108855624No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG2950.10610060495327978No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT2860.10286363734453563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)