FastQCFastQC Report
Sun 21 May 2023
EGAF00007833580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126443
Sequences flagged as poor quality0
Sequence length236-238
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT5954847.09473834059616No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1646913.024841232808459No Hit
GCTGCGTTCTTCATCGATGCAAGTGCACGTAAGCTGCGTTCTTCATCGAT58454.622636286706263No Hit
GCTGCGTTCTTCATCGATGCCGTCTATTTCGATAGGTTTCTCCATCACTT43583.4466123075219666No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG40303.1872068837341727No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG40233.1816707923728473No Hit
GCTGCGTTCTTCATCGATGAAGTGCACGTAAGCTGCGTTCTTCATCGATG26072.061798596996275No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT14951.1823509407400963No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT13741.0866556472086235No Hit
GCTGCGTTCTTCATCGATGCAAGTGCACGTAAGCTGCGTTCTTCATCGGC12270.9703977286208014No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT8860.7007109923048329No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT8580.6785666268595335No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT8450.6682853143313588No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA8020.6342778959689347No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG7860.621623972857335No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT7750.61292440071811No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC7600.6010613478009854No Hit
GCTGCGTTCTTCATCGATGAAGTGCACGTAAGCTGCGTTCTTCATCGGCT6970.5512365255490617No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG6110.4832216888242133No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT5530.4373512175446644No Hit
GCTGCGTTCTTCATCGATGCAGTGCACGTAAGCTGCGTTCTTCATCGATG4660.3685455106253411No Hit
GCTGCGTTCTTCATCGATGCCGCGCGCGGAGAATGCACCGACACAGCAGC4530.3582641980971663No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT4250.33611983265186685No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4210.3329563518739669No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT3910.3092302460397175No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3490.2760136978717683No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT3220.2546602026209438No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG3130.24754237087066902No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG2950.23330670737011935No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2840.22460713523089457No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2830.22381626503641958No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT2600.20562625056349504No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC2550.20167189959112009No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG2540.20088102939664512No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT2080.16450100045079602No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT2030.1605466494784211No Hit
GCTGCGTTCTTCATCGATGCGCTGCTGCGTCTCGGTCTTTGCGGCCGGAC1860.14710185617234645No Hit
GCTGCGTTCTTCATCGATGCCGTTGCCCAGCAGGCTCTCGGCCTGAGCCA1860.14710185617234645No Hit
GCTGCGTTCTTCATCGGCGCTTACTACCTAGGCATCCTTTGTGTGCCCTT1370.10834921664307237No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1370.10834921664307237No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG1360.1075583464485974No Hit
GCTGCGTTCTTCATCGATTCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1290.10202225508727253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)