FastQCFastQC Report
Sun 21 May 2023
EGAF00007833590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136167
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT135349.939265754551396No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT131589.663134239573465No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG128239.417112810005362No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG110858.140738945559496No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT69625.112839381054147No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG69265.086401257279664No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG65274.793378718779146No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT45433.336344341874316No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT42123.093260481614488No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG30402.232552674289659No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG30362.229615104981383No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG28522.0944869168006934No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGCTGAAAGTTG25281.856543802830348No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT23311.7118685143977617No Hit
GCTGCGTTCTTCATCGATGCAGACAACGATCAAATCCAAGCCGCCCATGA23301.7111341220706926No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT21941.6112567655893133No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC21901.6083191962810373No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT17431.280045826081209No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC16851.237451071111209No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCACCGTTTGTAGTTG14121.0369619658213811No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGATG12270.9010993853136223No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGAT12150.8922866773887946No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGCTGAAAGTTT11250.8261913679525876No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA11070.8129723060653462No Hit
GCTGCGTTCTTCATCGATGCTAGAGCCAAGAGATCCGTTGTTGAAAGTTT8430.619092731719139No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA8000.5875138616551734No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTT7140.5243561215272423No Hit
GCTGCGTTCTTCATCGATGCGGTCGCGGTCGGCGCAGTCCACTACGAAGA6960.5111370596400009No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA6900.506730705677587No Hit
GCTGCGTTCTTCATCGATACGAGAGCCAAGAGATCCACCGTTTGTAGTTG6660.4891052898279319No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT6360.4670735200158629No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG6350.46633912768879393No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG6000.44063539624138004No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTAAAAGTTT5950.4369634346060352No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTGAAAGTTG5910.43402586529775933No Hit
GCTGCGTTCTTCATCGATGCGCAAGCCAAGAGATCCGTTGTTGAAAGTTT5250.38555597171120753No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT4320.31725748529379366No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT4310.3165230929667247No Hit
GCTGCGTTCTTCATCGATGCTGGAGCCAAGAGATCCGTTGTTAAAAGTTT4290.31505430831258674No Hit
GCTGCGTTCTTCATCGATGCGAGGGCGTCGATTCCGGTTCACGCGCCGGC4280.3143199159855178No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG4030.2959601078087936No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT3650.26805319938017286No Hit
GCTGCGTTCTTCATCGATGTCCCCCGCTCTGCTGCGTCTTCTCCACCTCG3120.22913040604551765No Hit
GCTGCGTTCTTCATCGATGCGTTTTGGCAGCAAAATAGGCTTCTGGGTAT2800.20562985157931069No Hit
GCTGCGTTCTTCATCGATGCCCTATCAAGGACGCTGCGTTCTTCATCGCC2710.19902032063569003No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT2490.18286368944017273No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG2480.18212929711310374No Hit
GCTGCGTTCTTCATCGATGCACGAGGCGAGTGATCCACCGCTAAGAGTCG2460.18066051245896583No Hit
GCTGCGTTCTTCATCGATGCCTATCAAGGACGCTGCGTTCTTCATCGCCC2170.1593631349739658No Hit
GCTGCGTTCTTCATCGATGCGAGGGCTTCGATTCCGGTTCACGCGCCGGC2090.15348799635741406No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG2010.14761285774086233No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG1960.1439408961055175No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT1950.1432065037784485No Hit
GCTGCGTTCTTCATCGATGCCCTTGTGGCCACCCCCTGACATGACTCCTT1940.14247211145137956No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTAAAAGTTG1820.13365940352655198No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1760.12925304956413816No Hit
GCTGCGTTCTTCATCGATGCCGAGCCAGAAGTGATGGTTTTAGTGGGGAA1650.12117473396637951No Hit
GCTGCGTTCTTCATCGATGGCAGAGAGGGGGTCATTCATCCAGCTCTCCG1590.11676838000396572No Hit
GCTGCGTTCTTCATCGATGCACACATGGGGGCCACGTCAGGTGACCACTC1560.11456520302275883No Hit
GCTGCGTTCTTCATCGATGCTCATGAGACACTTTCATGAGCATTGATATC1460.10722127975206916No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCATTGTTATAAGTTG1460.10722127975206916No Hit
GCTGCGTTCTTCATCGATGCCTATGGCAATGGCTTGGAGAATAAAACCAG1430.10501810277086226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)