FastQCFastQC Report
Sun 21 May 2023
EGAF00007833611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402529
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA33970484.39242886847904No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC298597.417850639332818No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC80111.9901671680798154No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG25280.6280292848465576No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTTTCCGGCA8410.20892904610599486No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC5950.1478154369001985No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC5540.1376298353658991No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGGTGTGGTGTTCCAAGCAAGGCAGG5530.13738140606018448No Hit
GGAAGTAAAAGTCGTAACACGCCATACGGGTATTCGTGTTTTCGGAATGT5430.1348971130030383No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC5100.12669894591445585No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA4880.12123350118873422No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA4450.11055104104300559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)