FastQCFastQC Report
Sun 21 May 2023
EGAF00007833623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83160
Sequences flagged as poor quality0
Sequence length247-250
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5174262.219817219817216No Hit
GGAAGTAAAAGTCGTAACAAGGAATCATCATGCAGTGAGGAAGGATCATG69318.334535834535835No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA34134.104136604136603No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC28653.445165945165945No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG21902.6334776334776335No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC19822.3833573833573833No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT12131.4586339586339587No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC11651.400913900913901No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGCAGTCGGGGTACCAGTGCCCAGA7930.9535834535834536No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7280.8754208754208754No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC5780.6950456950456951No Hit
GGAAGTAAAAGTCGTAACAAGGCAGCAGTCTTGCCCTGAACATGGTTTCC4930.5928330928330928No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4860.5844155844155844No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCT4860.5844155844155844No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTAAAGGTTGCTCTCCATCTTGAG3110.37397787397787396No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC2680.32227032227032226No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA2650.3186628186628187No Hit
GGAAGTAAAAGTCGTAACAAGGGGAAGTGGGGGAGCGGGTCCTAGTGGAA2310.2777777777777778No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC2260.27176527176527177No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTACCGACCTCACCGTTGATGA1790.21524771524771524No Hit
GGACGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1690.20322270322270325No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1650.1984126984126984No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGATGGTGCTGAAGCCGTGTGTACAG1650.1984126984126984No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAGGGAGAGGACCTGGCCCAAGAAGG1630.196007696007696No Hit
GGAAGTAAAAGTCGTAACAAGTTTTCCGTAGGTGAACCTGCGGAAGGATC1540.1851851851851852No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1510.18157768157768156No Hit
GGAAGTAAAAGTCGTAACAAGCAGCTTGGTCCAGGGCGCCGCCCGAAGTG1470.17676767676767677No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA1440.17316017316017315No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1360.16354016354016354No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1160.13949013949013947No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1040.12506012506012507No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGACGGATC980.11784511784511784No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC900.10822510822510822No Hit
GGAAGTAAAAGTCGTAACAAGGATGGAAAGAGGTGGGGGAGGAGAAGAGG890.10702260702260702No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGATCGTGCAGCAAGGAATCATCATG880.10582010582010583No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)